Kladwang, W. et al. Nat. Chem. advance online publication (30 October 2011).
Kladwang et al. describe a 'mutate-and-map' strategy for inferring RNA structures, involving systematic mutagenesis of one nucleotide in a base pair, combined with high-throughput, single-nucleotide-resolution chemical accessibility mapping. This approach allowed them to model secondary structures of domains from diverse noncoding RNAs, including ribosomal RNA, ribozymes and riboswitches.
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'Mutate-and-map' RNA structure characterization. Nat Methods 8, 997 (2011). https://doi.org/10.1038/nmeth.1794
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DOI: https://doi.org/10.1038/nmeth.1794