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Improved vectors and genome-wide libraries for CRISPR screening

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Figure 1: CRISPR lentiviral vectors with higher functional titer.

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Acknowledgements

We thank the Zhang lab for support and advice. N.E.S. is supported by a postdoctoral fellowship from the Simons Center for the Social Brain at Massachusetts Institute of Technology. O.S. is supported by a postdoctoral fellowship from the Klarman Cell Observatory. F.Z. is supported by the US National Institutes of Health and NIMH through a Director's Pioneer Award (5DP1-MH100706); a Transformative R01 grant (5R01-DK097768); the Keck, Merkin, Vallee, Damon Runyon, Searle Scholars, Klingenstein and Simons Foundations; and by B. Metcalfe and J. Pauley.

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N.E.S., O.S. and F.Z. conceived of and designed the experiments. N.E.S. and O.S. performed the experiments and analyzed the data. N.S., O.S. and F.Z. wrote the manuscript.

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Correspondence to Feng Zhang.

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Competing interests

N.E.S., O.S. and F.Z. are on patent applications related to this work. F.Z. is a cofounder of and scientific advisor for Editas Medicine.

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Supplementary Figures 1–3, Supplementary Methods and Supplementary Data (PDF 638 kb)

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Sanjana, N., Shalem, O. & Zhang, F. Improved vectors and genome-wide libraries for CRISPR screening. Nat Methods 11, 783–784 (2014). https://doi.org/10.1038/nmeth.3047

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