Abstract
Single-particle cryo-electron microscopy (cryo-EM) has emerged over the last two decades as a technique capable of studying challenging systems that otherwise defy structural characterization. Recent technical advances have resulted in a 'quantum leap' in applicability, throughput and achievable resolution that has gained this technique worldwide attention. Here I discuss some of the major historical landmarks in the development of the cryo-EM field, ultimately leading to its present success.
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Acknowledgements
I would like to thank R. Glaeser for his feedback while writing this commentary. The author is supported by grants from the US National Institute of General Medical Sciences and is a Howard Hughes Medical Institute Investigator.
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Nogales, E. The development of cryo-EM into a mainstream structural biology technique. Nat Methods 13, 24–27 (2016). https://doi.org/10.1038/nmeth.3694
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DOI: https://doi.org/10.1038/nmeth.3694
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