A rapid mix-and-inject serial femtosecond crystallography approach enables structure determination of ligand-binding intermediates.
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References
Stagno, J.R. et al. Structures of riboswitch RNA reaction states by mix-and-inject XFEL serial crystallography. Nature http://dx.doi.org/10.1038/nature20599 (2016).
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Doerr, A. Blend-and-shoot crystallography. Nat Methods 14, 8–9 (2017). https://doi.org/10.1038/nmeth.4137
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DOI: https://doi.org/10.1038/nmeth.4137