Abstract
We found that glia secrete myoglianin, a TGF-β ligand, to instruct developmental neural remodeling in Drosophila. Glial myoglianin upregulated neuronal expression of an ecdysone nuclear receptor that triggered neurite remodeling following the late-larval ecdysone peak. Thus glia orchestrate developmental neural remodeling not only by engulfment of unwanted neurites but also by enabling neuron remodeling.
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Acknowledgements
We thank Y. Nakanishi, C. Hama, K. Ito, M.R. Freeman, the Bloomington Stock Center, the Vienna Drosophila RNAi Center, the Exelixis stock collection at Harvard Medical School and the Developmental Studies Hybridoma Bank for fly stocks and antibodies, M.A. Logan and C.-F. Kao for critical reading of the manuscript and members of the Lee laboratory for fruitful discussion. This work was supported by the US National Institutes of Health (NS42049 and MH83607 to T.L.).
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T.A. designed the study, conducted the experimental work, interpreted the data and wrote the manuscript. Y.H. and M.B.O. designed and established new transgenic lines. T.L. supervised the project and wrote the manuscript.
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The authors declare no competing financial interests.
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Supplementary Figures 1–13, Supplementary Table 1 and Supplementary Methods (PDF 878 kb)
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Awasaki, T., Huang, Y., O'Connor, M. et al. Glia instruct developmental neuronal remodeling through TGF-β signaling. Nat Neurosci 14, 821–823 (2011). https://doi.org/10.1038/nn.2833
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DOI: https://doi.org/10.1038/nn.2833
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