Abstract
Mutations in leucine-rich repeat kinase 2 (LRRK2) are the most common cause of familial Parkinson's disease. We found LRRK2 to be degraded in lysosomes by chaperone-mediated autophagy (CMA), whereas the most common pathogenic mutant form of LRRK2, G2019S, was poorly degraded by this pathway. In contrast to the behavior of typical CMA substrates, lysosomal binding of both wild-type and several pathogenic mutant LRRK2 proteins was enhanced in the presence of other CMA substrates, which interfered with the organization of the CMA translocation complex, resulting in defective CMA. Cells responded to such LRRK2-mediated CMA compromise by increasing levels of the CMA lysosomal receptor, as seen in neuronal cultures and brains of LRRK2 transgenic mice, induced pluripotent stem cell–derived dopaminergic neurons and brains of Parkinson's disease patients with LRRK2 mutations. This newly described LRRK2 self-perpetuating inhibitory effect on CMA could underlie toxicity in Parkinson's disease by compromising the degradation of α-synuclein, another Parkinson's disease–related protein degraded by this pathway.
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Change history
17 March 2013
In the version of this article initially published online, author Sheng-Han Kuo's name was misspelled. The error has been corrected for the print, PDF and HTML versions of this article.
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Acknowledgements
We thank the Michael J. Fox Foundation for kindly providing some of the LRRK2 antibodies, C. Calatayud for help with neuronal differentiation of iPSC, D. Arduino and B. Caballero for their help with the generation of the LRRK2 knockdown cells, J.P. Vonsattel and R. Alcalay for assistance with human tissue and A. Diaz and B. Patel for technical assistance in several aspects of this work. This work was supported by grants from the US National Institutes of Health National Institute on Aging (AG031782 and AG038072 (A.M.C.) and AG08702 (Alzheimer's Disease Research Center at Columbia University)), MINECO (SAF2012-33526, PLE2009-0144 and ACI2010-1117 (A.R.); RyC-2008-02772 and BFU2010-21823 (A.C.)), the Beatrice and Roy Backus Foundation (A.M.C.), the Rainwaters Foundation (A.M.C.), a Robert and Renee Belfer gift (A.M.C.), the JPB Foundation (D.S.), the Parkinson's Disease Foundation (D.S. and S.-H.K.), Fondazione Guido Berlucchi (A.C.), a CIBERNED Cooperative Project (A.R.), a US National Institute of Neurological Disorders and Stroke Udall Center of Excellence (A.M.C. and D.S.) and a Hirschl/Weill-Caulier Career Scientist Award (A.M.C.). S.J.O. was supported by US National Institutes of Health National Institute on Aging training grant T32AG023475 and S.-H.K. by an American Academy of Neurology Research Fellowship.
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S.J.O. performed most of the experiments, analyzed data and prepared a draft of the manuscript; S.-H.K. analyzed the human brain samples and conducted some of the experiments on mouse neuronal cultures; I.T. performed some studies in isolated rat liver and mouse, pull-down experiments and some analysis of the kinase inhibitors; E.A. performed studies in neuronal cultures; H.K. assisted with the CMA reporter analysis; I.F.-C., A.C. and A.R. were responsible for all studies with differentiated iPSC; E.C. and L.S.H. provided the brain samples; W.D. provided the LRRK2-expressing cells and advice on some aspects of the project; D.S. critically discussed the results, directed experiments in neuronal culture and human brain and edited and reviewed the final version of the manuscript; A.M.C. directed the study, designed most of the experiments and edited and reviewed the final version of the manuscript.
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Orenstein, S., Kuo, SH., Tasset, I. et al. Interplay of LRRK2 with chaperone-mediated autophagy. Nat Neurosci 16, 394–406 (2013). https://doi.org/10.1038/nn.3350
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DOI: https://doi.org/10.1038/nn.3350
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