Abstract
Circadian rhythms of behavior and physiology can be entrained by daily cycles of restricted food availability, but the pathways that mediate food entrainment are unknown. The dorsomedial hypothalamic nucleus (DMH) is critical for the expression of circadian rhythms and receives input from systems that monitor food availability. Here we report that restricted feeding synchronized the daily rhythm of DMH activity in rats such that c-Fos expression in the DMH was highest at scheduled mealtime. During food restriction, unlesioned rats showed a marked preprandial rise in locomotor activity, body temperature and wakefulness, and these responses were blocked by cell-specific lesions in the DMH. Furthermore, the degree of food entrainment correlated with the number of remaining DMH neurons, and lesions in cell groups surrounding the DMH did not block entrainment by food. These results establish that the neurons of the DMH have a critical role in the expression of food-entrainable circadian rhythms.
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Acknowledgements
We would like to thank Q. Ha and M. Ha for superb technical assistance. This research was supported by grants from the US National Institutes of Health to C.B.S. (HL60292) and J.J.G. (MH67413).
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Supplementary information
Supplementary Fig. 1
The daily rhythm of c–Fos in the DMH realigns with a restricted daytime meal. (PDF 388 kb)
Supplementary Fig. 2
DMH lesions block the preprandial rise in locomotor activity, body temperature, and wakefulness. (PDF 2156 kb)
Supplementary Fig. 3
Camera lucida drawings of ibotenic acid–induced lesions in the hypothalamus. (PDF 975 kb)
Supplementary Fig. 4
Lesions in the VMHdm do not abolish food entrainment. (PDF 538 kb)
Supplementary Fig. 5
Parabrachial nucleus lesions do not block food entrainment. (PDF 726 kb)
Supplementary Fig. 6
The DMH plays a critical role in the expression of SCN− and food–entrainable rhythms. (PDF 969 kb)
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Gooley, J., Schomer, A. & Saper, C. The dorsomedial hypothalamic nucleus is critical for the expression of food-entrainable circadian rhythms. Nat Neurosci 9, 398–407 (2006). https://doi.org/10.1038/nn1651
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DOI: https://doi.org/10.1038/nn1651
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