Abstract
The fluorescence characteristics of photoactivatable proteins can be controlled by irradiating them with light of a specific wavelength, intensity and duration. This provides unique possibilities for the optical labelling and tracking of living cells, organelles and intracellular molecules in a spatio-temporal manner. Here, we discuss the properties of the available photoactivatable fluorescent proteins and their potential applications.
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Acknowledgements
The authors are supported by the European Commission Framework Program 6 and the Russian Academy of Sciences for the programme in Molecular and Cell Biology (K.A.L., D.M.C. and S.L.), and the National Institute of General Medical Sciences and the National Institute on Drug Abuse (V.V.V.).
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Lukyanov, K., Chudakov, D., Lukyanov, S. et al. Photoactivatable fluorescent proteins. Nat Rev Mol Cell Biol 6, 885–890 (2005). https://doi.org/10.1038/nrm1741
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DOI: https://doi.org/10.1038/nrm1741
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