Abstract
Recently, a method to encode unnatural amino acids with diverse physicochemical and biological properties genetically in bacteria, yeast and mammalian cells was developed. Over 30 unnatural amino acids have been co-translationally incorporated into proteins with high fidelity and efficiency using a unique codon and corresponding transfer-RNA:aminoacyl–tRNA-synthetase pair. This provides a powerful tool for exploring protein structure and function in vitro and in vivo, and for generating proteins with new or enhanced properties.
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Acknowledgements
Work in the laboratory of P.G.S. is supported by the National Institutes of Health, the United States Department of Energy and the Skaggs Institute for Chemical Biology.
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Xie, J., Schultz, P. A chemical toolkit for proteins — an expanded genetic code. Nat Rev Mol Cell Biol 7, 775–782 (2006). https://doi.org/10.1038/nrm2005
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DOI: https://doi.org/10.1038/nrm2005
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