Abstract
In this work, we present the first NMR solution structure of a DNA/RNA hybrid containing stereoregular RP-phosphorothioate modifications of all DNA backbone linkages. The complex of the enzymatically synthesized phosphorothioate DNA octamer (all-RP)-d(GCGTCAGG) and its complementary RNA r(CCUGACGC) was found to adopt an overall conformation within the A-form family. Most helical parameters and the sugar puckers of the DNA strand assume values intermediate between A- and B-form. The close structural similarity with the unmodified DNA/RNA hybrid of the same sequence may explain why both the natural and the sulfur-substituted complex can be recognized and digested by ribonuclease H.
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Bachelin, M., Hessler, G., Kurz, G. et al. Structure of a stereoregular phosphorothioate DNA/RNA duplex. Nat Struct Mol Biol 5, 271–276 (1998). https://doi.org/10.1038/nsb0498-271
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DOI: https://doi.org/10.1038/nsb0498-271
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