Abstract
The natural versatility of RNA makes it an ideal substrate for bioengineering. Its structural properties and predictable base-pairing permit its use as molecular scaffold, and its ability to interact with nucleic acids, proteins and small molecules confers a regulatory potential that can be harvested to design RNA regulators in diverse contexts.
This is a preview of subscription content, access via your institution
Relevant articles
Open Access articles citing this article.
-
Scaling up genetic circuit design for cellular computing: advances and prospects
Natural Computing Open Access 05 October 2018
Access options
Subscribe to this journal
Receive 12 print issues and online access
$189.00 per year
only $15.75 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
References
Zappulla, D.C. & Cech, T.R. Proc. Natl. Acad. Sci. USA 101, 10024–10029 (2004).
Tsai, M.-C. et al. Science 329, 689–693 (2010).
SantaLucia, J. Proc. Natl. Acad. Sci. USA 95, 1460–1465 (1998).
Chworos, A. et al. Science 306, 2068–2072 (2004).
Severcan, I. et al. Nat. Chem. 2, 772–779 (2010).
Geary, C., Rothemund, P.W.K. & Andersen, E.S. Science 345, 799–804 (2014).
Delebecque, C.J., Lindner, A.B. & Silver, P. Science 333, 470–474 (2011).
Myhrvold, C., Dai, M. & Silver, P. Nano Lett. 13, 4242–4248 (2013).
Castellana, M. et al. Nat. Biotechnol. 32, 1011–1018 (2014).
Fu, J., Liu, M., Liu, Y., Woodbury, N.W. & Yan, H. J. Am. Chem. Soc. 134, 5516–5519 (2012).
Sachdeva, G., Garg, A., Godding, D., Way, J.C. & Silver, P.A. Nucleic Acids Res. 42, 9493–9503 (2014).
Chen, X. & Ellington, A.D. PLOS Comput. Biol. 5, e1000620 (2009).
Chen, Y.-J. et al. Nat. Methods 10, 659–664 (2013).
Na, D. et al. Nat. Biotechnol. 31, 170–174 (2013).
Takahashi, M.K. & Lucks, J.B. Nucleic Acids Res. 41, 7577–7588 (2013).
Green, A.A., Silver, P.A., Collins, J.J. & Yin, P. Cell 159, 925–939 (2014).
Gilbert, L.A. et al. Cell 154, 442–451 (2013).
Chen, Y.Y., Jensen, M.C. & Smolke, C.D. Proc. Natl. Acad. Sci. USA 107, 8531–8536 (2010).
Pardee, K. et al. Cell 159, 940–954 (2014).
Takahashi, M.K. et al. ACS Synth. Biol. doi:10.1021/sb400206c (28 March 2014).
Zadeh, J.N. et al. J. Comput. Chem. 32, 170–173 (2011).
Kosuri, S. et al. Nat. Biotechnol. 28, 1295–1299 (2010).
Ravikumar, A., Arrieta, A. & Liu, C.C. Nat. Chem. Biol. 10, 175–177 (2014).
Whitaker, W.R. & Davis, S. Proc. Natl. Acad. Sci. USA 109, 18090–18095 (2012).
Acknowledgements
We thank L. Liu, S. Hays and R. Chang for feedback while writing this commentary. Some of the work described here was funded by Defense Advanced Research Projects Agency award HR0011-12-C-0061 to P.A.S. C.M. is funded by the Fannie and John Hertz Foundation.
Author information
Authors and Affiliations
Corresponding authors
Ethics declarations
Competing interests
The authors declare no competing financial interests.
Rights and permissions
About this article
Cite this article
Myhrvold, C., Silver, P. Using synthetic RNAs as scaffolds and regulators. Nat Struct Mol Biol 22, 8–10 (2015). https://doi.org/10.1038/nsmb.2944
Published:
Issue Date:
DOI: https://doi.org/10.1038/nsmb.2944
This article is cited by
-
RNA origami design tools enable cotranscriptional folding of kilobase-sized nanoscaffolds
Nature Chemistry (2021)
-
Scaling up genetic circuit design for cellular computing: advances and prospects
Natural Computing (2018)
-
Engineering nucleic acid structures for programmable molecular circuitry and intracellular biocomputation
Nature Chemistry (2017)