Abstract
The zebrafish has emerged as a powerful genetic model of cancer, but has been limited by the use of stable transgenic approaches to induce disease. Here, a co-injection strategy is described that capitalizes on both the numbers of embryos that can be microinjected and the ability of transgenes to segregate together and exert synergistic effects in forming tumors. Using this mosaic transgenic approach, gene pathways involved in tumor initiation and radiation sensitivity have been identified.
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Acknowledgements
We thank Anna Burrows for expert technical help and David Bellovin and David Adamovich for critical review of this manuscript. DM Langenau is the Edmond J Safra Foundation Fellow from the Irvington Institute and CJ Ceol is supported by a Damon Runyon Fellowship (DRG-1855-05). Funding for this work was provided by NIH Grant 5R01 CA103846-02 (LI Zon), #1RO1 CA119066-01, CA93152-05, CA104605-03, HL-088664 and CA68484-11 (AT Look), #1KO1 DK074555-01 (CA Jette).
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Langenau, D., Keefe, M., Storer, N. et al. Co-injection strategies to modify radiation sensitivity and tumor initiation in transgenic Zebrafish. Oncogene 27, 4242–4248 (2008). https://doi.org/10.1038/onc.2008.56
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DOI: https://doi.org/10.1038/onc.2008.56
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