Abstract
Comparison of fractionation efficiencies of elution method and precipitation chromatography was made on a conventional polystyrene. Molecular weight distributions obtained from fractionation data were in close agreement with each other. The maximum molecular weight of these fractionations, however, was higher in the precipitation chromatography than in the elution method which indicates better resolution in the former than in the latter. But this difference was inverted when the elution method was applied to the identical sample but treated with acetylating agent. The molecular weight distributions of the representative fractions obtained by the two methods as measured by sedimentation velocity method at a theta condition were essentially symmetrical with respect to their centroid and had Mw/Mn values 1.04 to 1.06. These results obtained for fractions prepared by elution fractionation were qualitatively in agreement with the theoretical results, but those obtained by precipitation chromatography were far broader than that expected from the elementary consideration of the principle. Possible reasons of this discrepancy are considered.
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Ueda, M. Comparison of the Fractionation Efficiencies of Elution Method and Precipitation Chromatography of Polymer Fractionation. Polym J 3, 431–441 (1972). https://doi.org/10.1295/polymj.3.431
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DOI: https://doi.org/10.1295/polymj.3.431