Abstract
Uptake (U) and esterification (E) of oleic acid was studied in intact intestinal mucosa of infants and children. Crosby-Kugler intestinal biopsy specimens were incubated in vitro in Krebs-Ringer buffer (pH 6.3), 0.01 mM glucose, 2.4 mM Na taurodeoxycholate 0.06 μM oleic-1-14C, 0.54 μM oleic acid and 0.3 μM glycero-2-mon-opalmitate at 37°C, lipid extracted, separated by thin layer chromatography and counted in a scintillation spectrometer. All specimens were examined histologically. 26 patients (age 4 mos. to 14 yrs.) and 1 adult were studied in 4 groups. Group 1, functional diarrhea with normal mucosa, Group 2, treated celiacs and relatives, Group 3, patients with mucosal disease and Group 4, patients with intraluminal bile acid deficiencies were studied:
No significant difference in E or U with age was seen in Group 1. A simple reliable method to measure FA uptake and esterification in very small quantities of mucosa is presented. FA esterification, uptake and E/U were significantly depressed in mucosal disease and treated celiacs and their relatives. Application of the principles of this technique to other areas of mucosal metabolism may enable definition of previously undefined diarrheal states in infants and children.
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Heubi, J., Mcgraw, C., Partin, J. et al. 436 SMALL INTESTINAL FATTY ACID (FA) ESTERIFICATION IN INFANT AND CHILD MUCOSAL BIOPSIES. Pediatr Res 12 (Suppl 4), 436 (1978). https://doi.org/10.1203/00006450-197804001-00441
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DOI: https://doi.org/10.1203/00006450-197804001-00441