Abstract
Summary: Labeled choline incorporation into adult rat lung type II alveolar epithelial cells and adult rat lung fibroblasts in monolayer culture was dgtennined after incubation with insulin (Ins) 10 μg/ml. Dexamethasone (Dex) 10-6 M, or no drug (ND). Incubation periods were 1. 3. 4, and 5 hours. The lecithin (phosphatidyl choline - PC) recovered was separated into disaturated phosphatidyl choline (DSPC) and unsaturated phasphatidyl choline (USPC). Results expressed as specific activity per hour (see Table) indicate that the incorporatian of choline into PC and USPC was greater in fibroblasts (F) than in epithelial cells (E) whether ND, Dex or Ins was present. For incorporation into DSPC, there was no difference between E and F whether ND, Dex or Ins was present. There was significant increase in choline incorporation into PC or USPC for both cell types when Ins was present, whereas there was no difference for either cell type when Dex was present. Insulin significantly increased choline incorporation into DSPC in E cells only. Dex was no different from ND in DSPC incorporation in either cell type. We attribute the greater lecithin synthesis of the F cells to a more rapid increase in cellular strucrural lipids in the fibroblast cell. Dex had no effect an either cell type from the short-term exposure or possibly because the effect of dexamethasone on alveolar epithelial cells is mediated by product(s) from other lung cells. and thus requires a mixed cell culture to have its effect. We suggest that further study of isolated homogeneous cell lines vill not be fruitful in the evaluation of mechanisms of acceleration of lung maturation.
Speculation: Isolated alveolar epithelial cells shw a relatively greater increase in DSPC synthesis than do isolated fibroblasts in response to insulin. while neither cell type responds to short term glucocarticoid treatment. Since other investigators using mixed lung cell cultures have sham increased DSPC in response to insulin end even greater response with glucocarticoid treatment, it is likely that the presence of fibroblasts or other pulmonary cells is required for the alveolar epithelial cell to increase DSPC production in response to glucocorticoids.
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Sharp, M., Borer, R., Vadnay, L. et al. CHOLINE INCORPORATION INTO LECITHIN IN RESPONSE TO INSULIN OR DEXAMETHASONE IN HOMOGENEOUS CELL CULTURES OF RAT LUNG EPITHELIAL CELLS AND FIBROBLASTS. Pediatr Res 14, 899–900 (1980). https://doi.org/10.1203/00006450-198007000-00014
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DOI: https://doi.org/10.1203/00006450-198007000-00014
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