Abstract
Specific IB to erythrocytes was studied in 15 normal infants aged 1 to 24 months, and in 3 boys aged 2 to 20 months with nesidioblastosis 1/ at time of hyperinsulinism and hypoglycemia 2/ after correction of hypoglycemia by nasogastric feeding, diazoxide and/or partial pancreatectomy. In controls, IB ranged from 2.5 to 12.5 % and was significantly correlated with reticulocytes count : r = 0.824, p < 0.001. The addition of unlabelled Insulin decreased IB and scatchard analysis demonstrated 2 populations of sites with high (mean : 1.2 × 10-14M) and low (mean : 32 × 10-14M) affinity (for 3 109 erythrocytes), corresponding affinities being K = 5.5 × 109M-1 and 1.4 × 107M-1. In patients with nesidioblastosis, IB was elevated for reticulocytes count at the time of hypoglycemia in all cases (9 to 12 %) with an increased number of high affinity sites in 2. After treatment IB was low (2.5 to 4 %), the number of sites remaining identical but K being decreased. These data suggest that an increased receptivity to insulin may play a role in the mechanism of hypoglycemia in infants with hyperinsulinism.
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Garagorri, J., Donnadieu, M., Brijawi, A. et al. 125I-Insulin specific binding (IB) to erythrocytes of normal and hypoglycemic infants. Pediatr Res 15, 1547 (1981). https://doi.org/10.1203/00006450-198112000-00070
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DOI: https://doi.org/10.1203/00006450-198112000-00070