Abstract
Human blood mononuclear cells (MNC) lysed varicella zoster virus (VZV) infected targets in an 18 hour 51Cr release assay at effector:target (E:T) ratios of 50:1 to 10:1. Mean specific lysis at 50:1 E:T was 45% when effectors were autologous with targets; 35% when 2 or 3 HLA A or B antigens were shared and 18% when no HLA antiens were shared between E and T. Addition of antibody to HLA inhibited lysis by 65%. Inhibition of lysis following panning to deplete subsets selectively was: 52% for OKT 8; 44% for OKT 4; 30% for HNK 1 and OKM 1. Inhibition by OKT 4 was entirely, and by OKT 8 partly, reversed by addition of a PHA induced T cell supernatant (SUP). Density gradient seperated NK cells lysed targets in the presence of SUP. In combined depletion experiments the inhibition of lysis by anti-HLA summated with inhibition by HNK 1. The inhibition studies suggest the existence of at least two pathways for VZV-fibroblast lysis: one HLA restricted and presumably mediated by cytotoxic T lymphocytes and another dependent on lymphokine and mediated by NK cells. Studies in patients receiving leukemia remission maintenance treatment indicate that both pathways may be severely inhibited by chemotherapy.
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Bowden, R., Levin, M. & Hayward, A. CELLULAR INTERACTIONS IN THE LYSIS OF VZV INFECTED FIBROBLASTS. Pediatr Res 18 (Suppl 4), 253 (1984). https://doi.org/10.1203/00006450-198404001-00963
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DOI: https://doi.org/10.1203/00006450-198404001-00963