Abstract
Studies were undertaken to investigate the ability of the C8-substituted guanine ribonucleosides to modulate the proliferative and/or differentiative activity of T cells. This family of substituted nucleosides has been found to induce B cells to undergo proliferation and differentiation. However, T cells and thymocytes alike do not proliferate in response to 8MGuo. Moreover, this nucleoside does not modulate T cell proliferation evoked by either the mitogenic lectin Con A or by IL-2. 8MGuo can, however, modulate the T cell proliferative response to allogeneic cells. Because of earlier, vigorous B cell proliferation elicited by the nucleoside, modulation of T cell allogeneic responses must be observed in the absence of B cell responses. This can be done by stimulating thymocytes with irradiated cells and 8MGuo in the presence of supplemental IL-2, or by stimulating SJL spleen cells (whose B cells are hyporesponsive to 8MGuo) with irradiated allogeneic cells in the presence of 8MGuo. An analogous situation pertains to T cell function in that only certain functions can be modulated by 8MGuo. That is, T cells can not generate T cell growth or B cell differentiation activity in the presence of 8MGuo alone. Moreover, this nucleoside does not induce polyclonal cytotoxicity in T cell populations. It does, however, enhance generation of H-2-restricted CTL induced by allogeneic cells. Thus, in contrast to the situation pertaining to B cells, 8MGuo acts as an adjunct, but not as an initiator for T cell proliferation and differentiation.
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Goodman, M., Weiqle, W. ENHANCEMENT OF T CELL PROLIFERATION AND DIFFERENTIATION BY 8-MERCAPTOGUANOSINE: 70. Pediatr Res 19, 755 (1985). https://doi.org/10.1203/00006450-198507000-00090
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DOI: https://doi.org/10.1203/00006450-198507000-00090