Abstract
There are many reports indicating that in humans, many cells have adenosine receptors. In this experiment we studied the effects of methylthioadenosine(MTA), naturally occuring nucleosides, N6-phenylisopropyladenosine(PIA), a R site adenosine receptor agonist, and 2',5'-dideoxyadenosine(DDA), a P site adenosine receptor agonist, on polymorphonuclear leukocyte (PMN) function. PMN chemotaxis was examined by the modified Boyden Chamber method. PMN incubated with PIA showed no change in chemotaxis. In contrast, MTA and DDA reduced chemotaxis in a dose-dependent manner. The inhibition rate were 43.2% at 200 μM MTA and 20% at 200 μM DDA. Phagocytosis was determined by microscopic examination of PMN exposed to latex beads for 60 min at 37°C. PIA had no effect on phogocytic function over concentrations ranging from 20 μM to 1000 μM. In contrast, phagocytic function was markedly suppressed by DDA and MTA to 11.4% and 59.7% respectively at 500 μM. Superoxide(O /2)production by PMN, induced by 150 μg Con A and 1 g cytochalasin E, was measured by the reduction of cytochrome C. Cytochrome C reduction by PMN incubated with 100 μM MTA was reduced to 30% of the control value. 300 μM of PIA showed a slight decrease to 80% of the control value in cytochrome C reduction. These findings suggested that many functions of PMN are influenced by adenosine analogues.
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Nishida, Y., Takeuchi, A. & Miyamoto, T. Modulation of Polymorphonuclear leukocyte function by adenosine analogues: 140. Pediatr Res 19, 767 (1985). https://doi.org/10.1203/00006450-198507000-00160
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DOI: https://doi.org/10.1203/00006450-198507000-00160