Abstract
It is well established that growth-promoting effects of GH on target cells in vivo are mediated by a class of growth hormone-dependent polypeptides belonging to the group of somatomedins. We tested the effects of human GH (from France-Hypophyse) and purified IGF I/SMC in vitro on bone marrow and peripheral blood cells from children, by means of erythroid progenitor cloning technique, according to a miniaturized methylcellulose assay.
The erythropoietic proliferation was evaluated by the number of colonies derived from CFU-E (Colony Forming Units-Erythroid) and BFU-E (Burst Forming Units-Erythroid) and by the biochemical determination, in the cultured erythroblasts, of the activity of a cytosolic enzyme involved in the heme pathway (Uroporphyrinogen I synthase). In the presence of erythropoietin (0.5 IU/ml for CFU-E, 1 IU/ml for BFU-E) and fetal calf serum, human GH appeared to require very high concentrations (200-250 ng/ml) to stimulate the growth of CFU-E and BFU-E in vitro. In contrast, erythroid colony formation was significantly increased (p <0.05, Student's test) by as little as 1 ng/ml of IGF I/ SMC, with peak activities occuring with 10-50 ng/ml.
These results suggest that IGF I/SMC exerts a direct growth promoting effect on the erythroid lineage, among other growth factors and could be involved in the regulation of erythropoiesis.
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Claustres, M., Chatelain, P. & Sultan, C. COMPARATIVE EFFECTS OF HUMAN GROWTH HORMONE (GH) AND INSULIN-LIKE GROWTH FACTOR I/SOMATOMEDIN C (IGF I/ SMC) ON ERYTHROPOIETIC CULTURES FROM NORMAL CHILDREN. Pediatr Res 20, 1191 (1986). https://doi.org/10.1203/00006450-198611000-00106
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DOI: https://doi.org/10.1203/00006450-198611000-00106