Abstract
Leukocytes from normal term neonates, neonates with septicaemia and a high percentage of immature neutrophils and leukocytes from healthy adults were studied after separation. Micropipettes with a diameter of 5 μm were used to determine the pressure required to aspirate whole leukocytes. Smaller pipettes with a diameter of 2.7 μm were used to aspirate membrane/cytoplasma tongues from these cells. Lymphocytes and granulocytes required similar aspiration pressures although lymphocytes are smaller than granulocytes. Monocytes have large volumes and required correspondingly high aspiration pressures. Neonatal granulocytes, lymphocytes and monocytes tended to be larger and to require higher aspiration pressures than adult leukocytes. Neonatal bands and juvenile neutrolphils required very high aspiration pressures. At a constant aspiration pressure of 2 cmH2O, tongue formation in 2.7-μm pipettes was markedly slower for lymphocytes compared to mature granulocytes, with the values for immature granulocytes and monocytes lying between these two extremes. Tongue formation of neonatal and adult leukocytes followed similar patterns. We conclude that neonatal leukocytes increase vessel resistance more than adult cells due to immature leukocytes and the larger leukocyte size.
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Schneider, S., Zilow, E. & Linderkamp, O. ESTIMATION OF DEFORMABILITY OF NEONATAL AND ADULT LEUKOCYTES VIA MICROPIPETTE ASPIRATION. Pediatr Res 22, 219 (1987). https://doi.org/10.1203/00006450-198708000-00037
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DOI: https://doi.org/10.1203/00006450-198708000-00037