Abstract
Much attention is being directed to the incidence of growth hormone deficiency and limits of HGH-concentration in respons to stimuli are under debate. In this study 23 serum samples from four patients were obtained during various stimulation test and analyzed by means of the following kits: Pharmacia (RIA 100), CIS (58-HGH-RIA equal to Sorin HGHK-2), Serono (HGH, RIA) and Hybritech (Tandem-R-HGH). All results were obtained in mU/l, the first three kits being standardized against WHO 66/217 and the last against HS 2243 E NIH. Pharmacia RIA-100 was the “in house method” (x) and the following correlations between the results obtained by other methods (y) were obtained:
Samples of biosynthetic HGH: B-HGH was diluted to 22.1 mU/l and Pituitary standard P-HGH diluted to 18.8 mU/l, both from Nordisk Gentofte gave following results (mU/l):
In conclusion methods based on one antibody-RIA (CIS=Sorin, Serono) gave the highest levels of HGH in plasma samples. Of the two IRMA-kits, Hybritech with two monoclonal antibodies gave the lowest results. Accordingly the dividing line between normal and partial deficiency is closely linked to the HGH assay chosen.
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Damjær Nielsen, M., Kappelgaard, A., Dinesen, B. et al. 89 GROWTH HORMONE ASSAYS: CLINICAL RESULTS IS OBTAINED WITH COMMERCIAL KITS. Pediatr Res 24, 532 (1988). https://doi.org/10.1203/00006450-198810000-00110
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DOI: https://doi.org/10.1203/00006450-198810000-00110