Abstract
ABSTRACT: A major blood group antigenic epitope was identified on human pulmonary surfactant protein A (SPA). MAb and polyclonal antibodies generated against purified human SP-A aggregated blood group A human erythrocytes and immunostaine epithelial cells in a variety of human tissues, consistent with the tissue distribution of major blood group antigens. SP-A MAb (MAb-8) agglutinated red cells and immunostained tissues from A or AB blood groups, but did not react with cells or tissues from O or B individuals. MAb-8 immunostaining of tissue from blood group A individuals was ablated by incubation with blood group A red cells. MAb and polyclonal antibodies directed against A blood group antigens reacted strongly with purified SP-A obtained from a blood group A individual with alveolar proteinosis. MAb and polyclonal antibodies specific for B blood group antigen failed to react with SP-A from this patient or from patients who were in blood group B. Reactivity of anti-blood group MAb was lost after treatment of SP-A with endoglycosidase-F, demonstrating its reactivity with an epitope dependent on the asparagine-linked oligosaccharide at asparagine 187. Reactivity of MAb-8 with SP-A persisted after endoglycosidase-F treatment, but was lost after digestion with collegenase as assessed by Western blot after SDS-PAGE. Reactivity of MAb to SP-A was sensitive to β-elimination, supporting the presence of another blood group antigenic site distinct from the epitope dependent on the asparagine-linked carbohydrate. The finding that the SP-A molecule contains a major blood group epitope has implication for the clinical use of surfactant replacement preparations and diagnostic reagents based on this protein.
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Stahlman, M., Gray, M., Ross, G. et al. Human Surfactant Protein-A Contains Blood Group A Antigenic Determinants. Pediatr Res 31, 364–371 (1992). https://doi.org/10.1203/00006450-199204000-00012
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DOI: https://doi.org/10.1203/00006450-199204000-00012
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