We have previously demonstrated a CD18-dependent sequestration of neutrophils in the dog lung within 3 hrs after CPB cessation that contributes to pulmonary dysfunction (Circulation 1995;92:2276-2283). To determine if expression of ICAM-1 (a ligand for CD18) on pulmonary capillary endothelium contributes to this response, 6 adult mongrel dogs underwent 90 mins of hypothermic CPB (26-28°C) with 60 mins of aortic cross-clamp time. After warming, dogs were reperfused open chest for 9 hrs. Lung biopsies were obtained prior to CPB, 10 to 30 mins after CPB, and at 1,2,3,6 and 9 hrs thereafter. Northern blots of lung biopsy homogenates demonstrated an early induction of the ICAM-1 gene which persisted at 9 hrs. In-situ hybridization showed mRNA expression within the alveolar wall, on alveolar macrophages, on bronchial epithelial cells, and on vascular endothelium of arterioles and venules. Western blots showed significant ICAM-1 expression prior to CPB, and against this background little or no increase in ICAM-1 protein expression was discernible after CPB. Immunohistochemistry performed at the light microscopic level confirmed ICAM-1 expression within the alveolar wall but failed to show an increase post-CPB, and failed to distinguish alveolar epithelial from capillary endothelial expression. Immunoelectron microscopy detected an increase in capillary ICAM-1 expression in only 1 dog at 9 hrs of reperfusion. Our data suggest that pulmonary neutrophil sequestration early after CPB, although CD18-dependent, is independent of pulmonary capillary ICAM-1 expression.
Author information
Authors and Affiliations
Additional information
(Spon. by Jeffrey A. Towbin).
Rights and permissions
About this article
Cite this article
Dreyer, W., Kukielka, G., Burns, A. et al. INTERCELLULAR ADHESION MOLECULE-1 (ICAM-1) REGULATION IN THE CANINE LUNG AFTER CARDIOPULMONARY BYPASS (CPB). † 147. Pediatr Res 39 (Suppl 4), 27 (1996). https://doi.org/10.1203/00006450-199604001-00166
Issue Date:
DOI: https://doi.org/10.1203/00006450-199604001-00166