Abstract
We studied rates of granulocyte and platelets recovery in 359 consecutive subjects receiving blood cell infusions in the context of autotransplants for plasma cell myeloma (N = 216) and lymphomas (N = 143). Blood cells were mobilised with filgrastim given for 4–5 days and collected after a median of 2 (range, 1–2) apheresis. Apheresis products were stored at 4° C for a median of 3 days (range, 2–6 days). Most subjects received carmustine, etoposide, cytarabine and melphalan (BEAM), cyclophosphamide, carmustine and etoposide (CBV) or high-dose melphalan. Filgrastim was given post transplant to 319 subjects. Median numbers of mononuclear cells collected was 31 × 10E + 6/kg (interquartile range (IQR) 37 × 10E + 6 cells/kg). Median numbers of CD34-positive cells collected was 3.6 × 10E + 6/kg (IQR 3.8 × 10E + 6/Kg). Median viability after collection was 90% (IQR 7%) after storage, 88% (IQR 12%). A total of 255 of 256 evaluable subjects recovered bone marrow function and there was no late bone marrow failure. Median interval to neutrophils >0.5 × E + 9/L was 13 days (range, 9–39 days) and to platelets >20 × 10E + 9/L, 16 days (range, 7–83 days). These rates and ranges seem comparable to those reported after autotransplants of frozen blood cells. There was no correlation between numbers of storage days at 4 °C and viability afte storage (r = −0.018, p = 0.14)) nor rates of recovery of neutrophils (r = −0.054, p = 0.52) or platelets (r = 0.116, p = 0.14). Blood cells collected for autotransplant can be stored at 4 °C for 6 d. This method is simple, inexpensive and widely applicable.
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*Presented, in part, at the 56th Annual meeting of the American Society of Haematology, December 4-92014, San Francisco CA. Karduss-Urueta A, Ruiz-Arguelles GJ, Perez R et al. Cell-freezing devices are not strictly needed to start an autologous hematopoietic transplantation program: non-cryopreserved peripheral blood stem cells can be used to restore hematopoiesis after high dose chemotherapy: a multicenter experience in 268 autografts in patients with multiple myeloma or lymphoma. Study on Behalf of the Latin-American Bone Marrow Transplantation Group (LABMT). Blood 2014,124: 849.
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Kardduss-Urueta, A., Gale, R.P., Gutierrez-Aguirre, C.H. et al. Freezing the graft is not necessary for autotransplants for plasma cell myeloma and lymphomas. Bone Marrow Transplant 53, 457–460 (2018). https://doi.org/10.1038/s41409-017-0047-7
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DOI: https://doi.org/10.1038/s41409-017-0047-7
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