Abstract
The inherited retinal diseases (IRDs) have traditionally been described phenotypically with the description evolving to incorporate more sophisticated structural and functional assessments. In the last 25 years there has been considerable advances in the understanding of underlying genetic aetiologies. The role of the ophthalmologist is now to work in a multi-disciplinary team to identify the disease-causing genotype, which might be amenable to gene-directed intervention. Visual electrophysiology is an important tool to assist the ophthalmologist in guiding the clinical geneticist to reach a final molecular diagnosis. This review outlines the physiological basis for the ISCEV standard electrophysiology tests, the role of electrophysiology in localising the functional deficit, correlation with structural findings to guide diagnosis and finally management of IRDs in the era of genomics with emphasis on the outer retina.
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Introduction
The electroretinogram (ERG) has been a useful functional investigation used to interrogate different components of the afferent visual pathway for over a 100 years. With recent advances in retinal imaging, and the genomic revolution, the question arises, where does the ERG fit in the current diagnostic paradigm? In this review we will demonstrate the continued importance that the ERG plays in understanding the structure–function correlation in the diagnosis, natural history and monitoring treatment for inherited retinal disorders in the genomics era, with particular emphasis on the outer retina.
Retinal structure and function: the electroretinogram
In understanding the origins and cellular contributions of the retina that is recorded by the standardised ERG, the clinician will be assisted in determining the site of dysfunction in the visual pathway. Interpreting the ERG also requires an understanding of the retinal circuitry and the relative concentration and distribution of the individual elements. Cone photoreceptors comprise 5% of the total photoreceptors in the retina (4.6 million from a total 92 million) with 90% of the cones located in the retinal periphery [1]. The cones are crowded into the rod-free foveola at very high density facilitating our high visual acuity [2, 3]. Spatial acuity is low in the periphery due to pooling by peripheral retinal ganglion cells from photopic signals across extensive retinal areas [4].
The electrical potentials of the visual system are altered by stimulation with flashes of light or pattern variation, producing a mass response from the retina. Traces recorded from different electrodiagnostic procedures represent pooled cellular responses from different levels within the retina (Fig. 1). These traces are helpful in determining the objective function of the visual system, particularly when clinical phenotype does not correlate with multimodal imaging (MMI) or subjective function. These non-invasive procedures have been standardised by the International Society for Clinical Electrophysiology of Vision (ISCEV) so that electrophysiology results can be meaningfully interpreted and compared worldwide [5, 6].
Full-field ERG
Full-field ERG testing can help differentiate different types of photoreceptor dystrophies (rod–cone or cone–rod) and direct clinical geneticists in their investigations for a specific genetic diagnosis. The ISCEV standard full-field ERG (ffERG) gives a mass response of the retina under both scotopic and photopic conditions. The waveform recorded varies depending on the stimulation and recording conditions with the result being the sum of overlapping retinal pathway contributions [7,8,9] and is able to distinguish between generalised inner and outer dysfunction. The classic waveform (Fig. 2A) arising from bright-flash stimulation under dark- or light-adapted conditions is characterised by an electronegative a-wave representing hyperpolarisation of the photoreceptors’ inner segments and the b-wave arising from the inner retina, predominantly the bipolar cells with a contribution from Muller cells. The oscillatory potentials are thought to be generated by post-receptor dopamine pathways with a presumed significant contribution from amacrine cells [10] (Fig. 2).
Patients need “dark adaptation” of at least 20 min and a dim light stimulus below cone threshold, to test the scotopic system, as rods are 1000 times more sensitive to light than cones. The response of the very dim light (DA 0.01) has an almost undetectable a-wave but a prominent b-wave, which is the response of the rod bipolar cells collectively. This dim flash tests the sensitivity of the scotopic system (Fig. 2B) and anatomically, an abnormal response to this weak flash stimulus is either due to rod pathway dysfunction or that of the rod bipolar cell. Increasing the intensity of the light stimulus to DA 3.0 and DA 10.0, incorporates a response that includes both rod and cone systems. Under scotopic conditions the rod system dominates the final waveform with the a-wave representing rod photoreceptors [11], the a-wave slope phototransduction kinetics [12, 13] and the b-wave the rod bipolar cells [11] (Fig. 2C). The ISCEV standard strong flash [5] is DA 10.0 cd s m−2, the standard allows for some variability in the clinical case examples presented in this paper reports responses from the strong flash stimulus of DA 12.0 cd s m−2, which is minimally different in log sensitivity terms and is interchangeable. Figures in this review use traces representing the DA 12.0 response.
The photopic system is tested following light adaptation for 10 min at a background luminance of 30 cd m−2. The rod system is suppressed by this luminance and by the 30 Hz flicker stimulus as rods only respond up to 20 Hz stimulus [7, 14]. The 30 Hz flicker (LA 30 Hz) is the most sensitive measure of cone system function, however it does not distinguish between cone photoreceptor and inner retinal layer connection dysfunction (Fig. 2B). The single flash (LA 3.0) stimulus is used to elicit specific components of the photopic system with the single flash (LA 3.0) a-wave reflecting the response of the hyperpolarising off-bipolar cells and cone photoreceptors [15], whilst the b-wave has contributions from both on- and off-bipolar cells [6, 7, 16, 17] (Fig. 2C). The standard flash (3.0 cd s m−2) ERG is specific for the location in the cone system.
The ffERG is a measure of the entire retinal function, which means that loss of function from focal lesions, including the macula, is averaged with the remainder of the retinal function and the focal functional significance is often not demonstrated. To address this issue ISCEV standard topographical retinal testing has been developed. The available tests are the pattern ERG (PERG) [18] and the multifocal ERG (mfERG) [19] (Fig. 3). Assessing macular function is important for diagnostic and functional assessments. The PERG and mfERG achieve this in different ways. Optimal refraction is required for both tests as well as fixation. The PERG is performed with undilated pupils but the mfERG protocol requires pupil dilation and supplementary lenses. Pattern ERG tests average macular function to a set stimulus field size, whereas the mfERG response demonstrates a topographic local macular response to a local luminance change in the hexagon display.
Pattern ERG (PERG)
The PERG waveform is the average of several hundred alternating stimulus responses and forms a discrete response with a positive and negative component (Fig. 3B). The positive deflection of the waveform is at a latency of approximately 50 ms and is referred to as P50. The larger negative component, N95, has a latency of approximately 95 ms (Fig. 3B). In contrast to the ffERG, the PERG stimulates a smaller area of retina and the resultant response has a lower amplitude. Averaging is needed to improve the signal-to-noise ratio with sometimes more than 100 trials per average is required. The N95 component of the human PERG is a contrast-related component generated in relation to the retinal ganglion cells [20, 21]. The PERG P50 component has its partial origins in the inner retina, but depends on the functional integrity of the macular photoreceptors and is used as an objective measure of preganglionic macular function [22]. Good, steady fixation is required by the patient, as with the mfERG. Doubling the stimulus size from 15° to 30° can assess the paramacular region [22, 23]. The larger field helps distinguish between localised central, predominantly paracentral, and widespread macular dysfunction. This provides an alternative to a multifocal ERG when precise steady fixation is not possible [22].
Multifocal ERG (mfERG)
The mfERG provides a topographic measure of retinal electrophysiological activity by stimulating localised areas of the central 45° or 60° field. The stimulus is an array of hexagonal elements. With this technique, many local ERG responses, typically 61 or 103, are recorded from the cone-driven retina under light-adapted conditions. The size of the hexagons of the ISCEV mfERG standard are scaled to gain comparable response amplitudes resulting in smaller hexagons for the central most stimuli size (Fig. 3D) [19]. Each element follows the same pseudo-random sequence of illumination. Cross-correlation of the continuous ERG signal with the sequence of on- and off-phases of each element enables calculation of an ERG signal at each location. The mfERG waveform is biphasic with an initial negative N1, a response from cones and their bipolar cells, and a later positive P2 component from the cone bipolar cells (Fig. 3E) [24, 25]. Good fixation is required to ensure topographic mapping (Fig. 3F) and a visual acuity of better than 6/60 is required. The mfERG is useful in identifying topographical location of retinal pathology up to 50° centred on the fovea.
Electro-oculogram (EOG)
The Electro-oculogram (EOG) assesses generalised retinal pigment epithelium (RPE) function as there is a potential difference between the basal and apical surface of the RPE of about 60 mV. The RPE itself is not a light receptor, however activation of the photoreceptors leads to a change in the ionic gradients across the RPE. The EOG is recorded during 15 min of dark adaptation followed by a further 15 min of light adaptation. The recordings are made with the eye (a dipole) making horizontal movements (saccades) from left to right. After about 7–10 min the dark trough (DT) (the lowest resting potential) is recorded. Similarly after about 7–10 min of light adaptation, the resting potential reaches its highest, known as the light peak (LP). This rise is due to the change in potassium currents of the photoreceptor–RPE matrix [26]. The ratio of LP/DT, termed the Arden ratio, provides a measure of function of this photoreceptor–RPE complex: normal is greater than 1.7 [26] (Fig. 4A). Clinically, if the photoreceptors are dysfunctional then the EOG will also be affected [26]. There are a small group of disorders with a normal ffERG and an abnormal EOG. The most important is Best vitelliform macular dystrophy (Fig. 4B–D).
Visual electrophysiology in the clinic
The care of medical retina patients (e.g. diabetic retinopathy, age-related macular degeneration, autoimmune retinopathy), inherited retinal diseases (IRDs) and diagnostic dilemmas increasingly require objective functional assessments. Questions of when to refer and what tests to choose are now common clinical questions. Visual electrophysiology referrals are recommended to assist in establishing a clinical diagnosis and in determining the level of visual function [6]. Further roles arise from these investigations in adding the establishment of a genetic diagnosis and monitoring the natural history [27].
The paediatric patient also requires objective functional vision assessment; particularly when there are concerns regarding visual development or a nonvisual infant, a family history of poor vision, or systemic or metabolic disease.
Younger children are not capable of undertaking a full-length ISCEV protocol, however a meaningful electrophysiological examination that is comparable to the ISCEV standard. These modifications include shorter dark adaptation, modified protocols and the use of less invasive skin electrodes [5]. The recordings from skin electrodes have smaller amplitudes but similar waveforms to those of gold foil electrodes. Many paediatric electrophysiology testing centres are able to study young children without sedation or general anaesthesia, using swaddling, patience and teamwork.
For both adults and children, the specific electrophysiology tests performed will be directed by the clinical presentation, incorporating a combination of history, examination and ocular investigations. The clinical history pertinent to IRDs include symptoms of the following: rod dysfunction (nyctalopia and/or peripheral vision issues), cone dysfunction (photophobia, reduced distance and near visual acuity and/or dyschromatopsia), age at onset and progression of symptoms [27,28,29]. IRDs can also occur as part of a multi-system condition and systemic medical problems need to be specifically inquired about and evaluated, particularly hearing, renal dysfunction, neurological dysfunction, skeletal anomalies and metabolic disturbance. The effects of medication also need to be evaluated [30].
The clinical question being asked determines the initial visual electrophysiology tests to be performed. When differentiating whether a patient has poor cone function, or no cone function the ffERG is helpful (Fig. 5A–F). If determining whether a patient has a retinopathy or a maculopathy the addition of the PERG ± mfERG to the ffERG is required (Fig. 6A, B). Poor central vision requires a pattern visual evoked potential (VEP) to exclude an optic neuropathy, which may be performed simultaneously with a PERG to determine whether there is a macular dysfunction and a ffERG to confirm whether there is generalised retinal dysfunction. Nyctalopia requires a ffERG to identify rod dysfunction, determine whether the nyctalopia is likely to be progressive or stationary and a PERG to determine residual macular function (Fig. 7).
In rod–cone dystrophies (retinitis pigmentosa, RP), secondary cone degeneration follows rod degeneration centripetally and is slowest at the macular where cone density is greatest. This correlates with the clinical finding of central visual acuity preservation until advanced stages. Measuring the rate of cone degeneration with the ffERG provides one measure of RP progression. The “fused” flicker amplitude (FFAmp) is a non-ISCEV standard waveform that enables assessment of residual cone function, described by Berson et al. [31]. The electrophysiology system records at the frequency of 30 ± 1 Hz and improves the signal-to-noise ratio by computer averaging 1200 trials over 10 min enabling amplitudes as low as 0.05 μV to be recorded with Burian Allen electrode. The more common clinically used electrodes (gold foil and DTL) may only record to 1 μV [31]. The importance of recording FFAmp is that useful vision is retained even when the cone function drops below 10 μV and is maintained until 0.05 μV. Below this critical level, virtual blindness ensues. The rate of decline in both 30 Hz flicker amplitude and FFAmp provide a guide to predict maintenance of useful vision [31, 32].
Inherited retinal disorders (IRD) with pathognomonic ERGs with phenotype–genotype correlation
Investigations with an ERG establishes whether a patient has a retinal or macular dysfunction and what anatomical level it is. In conjunction with the patient’s symptoms and MMI, this dysfunction can be classified as a probable dystrophy, facilitating a more directed clinical genetic workup within a genetic multi-disciplinary team (MDT).
Several IRDs have specific visual electrophysiology changes that are pathognomonic and correlate with genotype [33]. These include KCNV2 retinopathy, congenital stationery night blindness, enhanced S-cone syndrome and bradyopsia.
Cone dystrophy with supernormal rod ERG
KCNV2-associated retinopathy (cone dystrophy with supernormal rod responses) first described by Gouras et al. [34] is an autosomal recessive (AR) cone–rod dystrophy caused by mutation in the KCNV2 gene and has characteristic ERG findings. This gene encodes Kv8.2, a voltage-gated potassium channel, which sets the vertebrate photoreceptor resting potential and voltage-gated response [35].
Affected individuals usually present in the first two decades of life with poor visual acuity, abnormal colour vision and variable night vision difficulties [36]. Most patients are myopic and nystagmus is common. The fundus appearance can appear normal in the early stages, but later there may be macular pigmentary disturbance or macular atrophy (Fig. 8B–D).
The ERG (Fig. 8A) shows a markedly delayed rod ERG (DA 0.01). The arrow highlights the marked delay in b-wave implicit time compared to the normal control (same time scale) red dotted line indicates mean normal implicit time. The strong flash (DA 10.0) reveals a normal a-wave slope and normal or near normal amplitude with a broadened flat trough with a “squared” or “rhomboid” shape [37], with a steeply rising high amplitude b-wave (* in Fig. 8) best seen in the dark-adapted (DA) maximal (DA 12) recording. Photopic flicker and single flash ERGs are significantly reduced [36, 37]. Additional non-ISCEV standard luminance response series in the dark-adapted phase are useful in the diagnosis of this condition [38]. The KCNV2 transcript has been demonstrated in the inner segments of both cone and rod photoreceptors, which might explain the typical ERG findings in keeping with dysfunction occurring after phototransduction but before generation of b-wave [39].
Enhanced S-cone syndrome (ESCS)
Enhanced S-cone syndrome first described by Marmor et al. [40] is an AR disorder characterised by early onset night blindness, maculopathy with variable loss of visual acuity and increased sensitivity to blue light caused by mutations in NR2E3 gene [41]. NR2E3, a nuclear receptor specifically expressed in photoreceptors, has a key role in rod and cone photoreceptor development and maintenance by repressing cone-specific genes and activating several rod-specific genes. Mutations lead to excess S-cone proliferation at the expense of other cones/rods (abnormal cell fate determination) [42]. Mutations in NR2E3 gene have also been identified in Goldmann-Favre syndrome [43, 44].
Patients with ESCS present with nyctalopia with or without reduced VA in early childhood usually in the first decade of life [41, 45]. Posterior segment examination reveals optically empty vitreous, typical nummular pigmentary changes at the level of RPE along or outside the vascular arcades, macular schisis which is more obvious on spectral domain optical coherence tomography (SD-OCT) and sometimes whitish retinal deposits or hyperpigmented lesions (Fig. 9D). Thickening of the outer nuclear layer (ONL) on SD-OCT is another common structural finding [46, 47]. Fundus autofluorescence (FAF) shows partial or complete loss of autofluorescence (AF) outside the arcade presumably due to photoreceptor loss (Fig. 9C) [48].
Patients with ESCS show characteristic full-field ERG waveform abnormalities. The dark-adapted (DA) 0.01 response (rod specific) is bilaterally undetectable. The DA 3.0 and light-adapted (LA) 3.0 responses have the same simplified waveform and are markedly delayed. The 30 Hz flicker electroretinography is both delayed and of lower amplitude than the LA 3.0 response a-wave (Fig. 9A). S-cone-specific testing provides further confirmation of the diagnosis. The S-cone ERG is elicited using short-wavelength (blue) flashes on a long-wavelength background to saturate the rod system and to reduce contributions from the L/M-cone systems. The S-cones are isolated using a 5-millisecond duration short-wavelength (blue) stimulus (445 nm, 80 cd m−2) superimposed on a long-wavelength background (orange, 620 nm, background luminance 560 cd m−2) to saturate the rod system and to reduce contributions from the L/M-cone systems [49, 50]. These are typically larger than normal and resemble the waveform of LA 3.0 ERG due to the predominant contribution of S-cones to both these responses. PERG abnormalities vary from being reduced and/or delayed or completely unrecordable [41].
Bradyopsia
Bradyopsia is a rare stationary form of retinal dysfunction due to biallelic mutations in the gene RGS9 (regulator of G-protein signalling 9) or R9AP (regulator of G-protein signal 9-anchoring protein). It was first described by Kooijman et al. [51], and the term bradyopsia (“slow vision”) coined by Nishiguchi et al. [52]. RGS9 facilitates the hydrolysis of GTP (α-transducin bound) to GDP and ultimately opening of cGMP-gated cation channels in darkness [52]. Mutations in these genes impair the retina’s ability to rapidly adjust to and from bright light by slowing guanosine triphosphate (GTP) hydrolysis [53].
Patients with this condition have photophobia, mildly reduced visual acuity, difficulty tracking moving objects, trichromatic colour discrimination and delayed light/dark adaptation [52, 54,55,56]. Vision may be better under reduced luminance conditions and improve with pinhole [55]. Fundus examination and macular OCT are normal.
The ISCEV standard ERGs will often misdiagnose bradyopsia. The PERGs are usually undetectable. The DA 0.01 ERG is normal and DA 3.0 ERGs are often normal to only mildly reduced in amplitude (Fig. 10). The photopic ERGs (30 Hz and LA 3.0) are undetectable or profoundly subnormal. The ISCEV DA red flash ERG extended protocol presents a red flash under scotopic conditions [57]. The resultant waveform normally has two distinct positive peaks, the first positive component is the cone-mediated x-wave with a peak at 30–50 ms; the second is a rod-mediated b-wave with a peak time of approximately 100 ms. The combination of a preserved DA red flash ERG x-wave and undetectable or severely abnormal standard LA ERGs is pathognomonic for bradyopsia [33, 53, 57].
Electrodiagnostic role in genetic diagnosis
IRDs caused by monogenic gene mutations are the commonest cause of blindness in young and working age individuals in many countries causing a huge economic burden [58, 59]. In the 1990s the primary goal of the ophthalmologists with visual electrophysiology information and Clinical Geneticists was to give the IRD a name, try to establish an inheritance pattern and estimate recurrence risk for other family members. Molecular diagnosis was a challenge since the genetic testing was either single gene based or a chip approach searching for a limited number of known gene mutations.
Next-generation sequencing technology has enabled vastly improved mutation detection rates. The advances have also highlighted variants of uncertain significance (VUS) that are simultaneously identified [27]. The American College of Medical Genetics and Genomics (ACMG) provides a systematic methodology to classify these variants [60].
Advances in understanding the cell biology of the visual system, gene replacement approaches, and CRISPR-based genome editing have led to successful use of genetic therapies for IRDs [61] and multiple clinical trials are now underway across a spectrum of conditions. In the genomics era, the multi-disciplinary team now consists of input from ophthalmologists with visual electrophysiology information, clinical geneticists and genomic laboratory scientists, and the clinical goal is strongly focussed on identifying the disease-causing genotype and evaluating for therapy suitability.
Electrophysiology contributing to MDT genetic review
Pathogenic variants in more than 250 genes can give rise to IRDs with multiple modes of inheritance [62]. However, there exists a considerable genetic and phenotypic heterogeneity [63]. Due to this, prioritisation in examining the genes known to be associated with a certain IRD is challenging.
A genetic MDT approach where the ophthalmologist liaises with the clinical genetics team is extremely important in the diagnosis and appropriate management of IRDs. Visual electrophysiology has two broad roles in assisting genetic diagnosis. Firstly, the electrophysiology provides functional results that assist in localisation of the defect to a particularly retinal cell type and thus a diagnostic group. This information guides the clinical geneticist in selecting gene panels that encompass the most common genetic causes for the group. Secondly, structure–function outcomes obtained with visual electrophysiology assists in interpreting molecular genetic results. This commonly involves confirming that the identified molecular genetic change is consistent with the patient phenotype. Importantly the electrophysiology assists in interpreting variants of uncertain significance (VUS).
Electrodiagnostic testing as a guide for genetic panel screening
Multigene panel testing is a well-established approach to dissect genetically heterogenous diseases and has been a validated method for clinical diagnosis [64,65,66]. Application of a focussed approach to gene panel testing can increase the chance of the correct molecular diagnosis and reduce the presence of confounding variants of uncertain significance (VUSs). Electrodiagnostic tests can help guide this testing. As an example, an abnormal PERG ± mfERG with normal ffERG will help direct the genetic panel testing to a maculopathy as against a generalised retinopathy. These of course, need to be considered in the context of clinical presentation, family history and MMI.
A “bulls-eye” maculopathy is a relatively common clinical presentation and is frequently seen in cone/cone–rod dystrophies. However, as with the example in Fig. 11, other IRDs can have the phenotype of a bulls-eye maculopathy, but functional studies demonstrate a different diagnosis. The ffERG of Fig. 11 is consistent with achromatopsia and this information would lead to an expansion of the gene panel being evaluated so that a CNGB3 mutation may be correctly identified.
Patients with retinitis punctata albescens (RPA) and fundus albipunctatus (FA) present with nyctalopia and similar fundal phenotype with whitish, glistening small deposits across the outer retina (white fleck syndrome) (Fig. 12). Distinguishing the two diagnosis functionally with electrophysiology again guides genomic diagnosis. RPA is a subtype of RP with a similar progressive time course, and in most cases is caused by pathogenic variants in RLBP1 gene [67, 68]. Electrophysiology on these patients demonstrates a rod–cone dysfunction (Fig. 12, left ffERG panel). In contrast, FA is a recessively inherited, usually stationary disorder [69] and is most commonly associated with RDH5 gene mutations [70]. Bright-flash dark-adapted DA 12.0 ERG of patients with FA have subnormal a-wave and reduced b-wave amplitude commonly resulting in an electronegative ERG appearance (Fig. 12, right ffERG panel). Repeating the ffERG following an extended period of dark adaptation (prolonged dark adaption), the DA 0.01 and DA 10.0 ERGs wave form returns to normal (red box Fig. 12) [71]. Prolonged dark adaption is performed by patching and occluding one eye overnight and then performing standard ISCEV dark-adapted ffERG with the patch taken off after the 20 min of further dark adaption. This enables the unpatched (control eye) and the prolonged dark adaption to be compared. Demonstration of this electrophysiology pattern helps the ophthalmologist give prognosis to the patient and help guide clinical geneticists with their genetic panel screen.
Electrodiagnostic testing role in assessing molecular genetic results including variants of uncertain significance (VUS)
Genetic testing to identify the cause of disease has become increasingly important as more clinical trials for IRD focus on patient populations with specific genotypes [72], for example, studies recruiting USH2A, RPGR, CHM, ABCA4, MERTK, CNGA3, CNGB3, PDE6B or RLBP1‐ affected patients (https://clinicaltrials.gov/). In addition, IRD patients due to RPE65 mutations would be eligible for the first Food and Drug Administration (FDA)‐approved gene therapy, voretigene neparvovec (Luxturna) [73]; Patients without a genetic diagnosis are not eligible for gene‐specific treatments.
IRD genetic testing is the same as other panel-based genomic testing strategies in that candidates with VUS are often identified with insufficient evidence to deem as disease causing, or in genes which have not been described in the literature matching the particular phenotypic retinal presentations. Incorporating an ocular genetics MDT approach can improve diagnostic rates due to clarification of ocular phenotype review of clinical features in family members, and segregation studies of candidate genetic variants. Visual electrophysiology contributes important objective functional phenotypic information to the MDT review process.
Conclusions
Visual electrophysiology plays an important role in the management of IRDs across all stages of their management from diagnosis, determination of retinal and visual function, guidance for genomic testing, interpretation of genomic results and monitoring visual function over time as part of natural history evaluation.
Methodology
We used the following databases and search terms to research this review: MEDLINE/PubMed: Inherited Retinal Degeneration; Electrophysiology; ERG; Pathognomonic ERG; Enhanced S Cone Syndrome; KCNV2 retinopathy; Bradyopsia; ISCEV standard ERG; full-field electroretinogram; pattern electroretinogram; multifocal electroretinogram; ABCA4 associated retinal degeneration; Genetic basis of Inherited Retinal disease; Molecular genetics in inherited retinal disorders; Genomic applications in diagnosis of retinal dystrophies; gene therapy for retinal dystrophies. Non-English papers were excluded.
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J.G., E.C., A.V. and R.J. conceived and designed the work. J.G., E.C., A.V. and R.J. drafted and revised the manuscript. J.G., E.C., A.V. and R.J. approved the final version. J.G., E.C., A.V. and R.J. agreed to be accountable for all aspects of the work in ensuring that questions related to the accuracy or integrity of any part of the work are appropriately investigated and resolved.
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Cornish, E.E., Vaze, A., Jamieson, R.V. et al. The electroretinogram in the genomics era: outer retinal disorders. Eye 35, 2406–2418 (2021). https://doi.org/10.1038/s41433-021-01659-y
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DOI: https://doi.org/10.1038/s41433-021-01659-y
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