Fine-tuning gene expression is crucial for generating quantitative phenotypic changes and crop improvement. Using CRISPR–Cas base editing and prime editing, we engineered upstream open reading frames — eukaryotic translational control elements — in rice to incrementally downregulate translation to predictable and desired levels.
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References
Gao, C. Genome engineering for crop improvement and future agriculture. Cell 184, 1621–1635 (2021). A review of the development and application of genome editing tools in plants.
Bowman, E. K. et al. Bidirectional titration of yeast gene expression using a pooled CRISPR guide RNA approach. Proc. Natl Acad. Sci. USA 117, 18424–18430 (2020). A plasmid-based library methodology for bidirectional, graded modulation of gene expression in yeast.
Rodríguez-Leal, D. et al. Engineering quantitative trait variation for crop improvement by genome editing. Cell 171, 470–480.e478 (2017). A way to dissect the biology of quantitative trait loci by targeting promoters using CRISPR–Cas9.
Zhang, H. et al. Genome editing of upstream open reading frames enables translational control in plants. Nat. Biotechnol. 36, 894–898 (2018). A method for upregulating protein expression by editing uORFs.
Zhang, T., Wu, A., Yue, Y. & Zhao, Y. uORFs: important cis-regulatory elements in plants. Int. J. Mol. Sci. 21, 6238 (2020). A review article describing the roles of uORFs in modulating plant growth, development and environmental adaptation.
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This is a summary of: Xue, C. et al. Tuning plant phenotypes by precise, graded downregulation of gene expression. Nat. Biotechnol. https://doi.org/10.1038/s41587-023-01707-w (2023).
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Engineered upstream open reading frames predictably downregulate mRNA translation in plants. Nat Biotechnol 41, 1694–1695 (2023). https://doi.org/10.1038/s41587-023-01729-4
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DOI: https://doi.org/10.1038/s41587-023-01729-4
