An NMR method to monitor conformational states of challenging large protein targets is described. The method, which can be used to evaluate distances between two labels and to measure conformational exchange rates, revealed an unanticipated outward-facing state in a glutamate transporter.
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References
Henzler-Wildman, K. & Kern, D. Nature 450, 964–972 (2007).
Huang, Y. et al. Nat. Chem. Biol. https://doi.org/10.1038/s41589-020-0561-6 (2020).
Cheng, Y. Science 361, 876–880 (2018).
Sahu, I. D. & Lorigan, G. A. Biomolecules 10, 763–788 (2020).
Mazal, H. & Haran, G. Curr. Opin. Biomed. Eng. 12, 8–17 (2019).
Di Pietrantonio, C., Pandey, A., Gould, J., Hasabnis, A. & Prosser, R. S. Methods Enzymol. 615, 103–130 (2019).
Rose-Sperling, D., Tran, M. A., Lauth, L. M., Goretzki, B. & Hellmich, U. A. Biol. Chem. 400, 1277–1288 (2019).
Pavić, A., Holmes, A. O. M., Postis, V. L. G. & Goldman, A. Biochem. Soc. Trans. 47, 1197–1207 (2019).
Tanford, C. Annu. Rev. Biochem. 52, 379–409 (1983).
Sekhar, A. & Kay, L. E. Annu. Rev. Biophys. 48, 297–319 (2019).
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Cheng, R.C., Maduke, M. Expanding the membrane-protein NMR toolkit. Nat Chem Biol 16, 937–938 (2020). https://doi.org/10.1038/s41589-020-0597-7
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DOI: https://doi.org/10.1038/s41589-020-0597-7