Bioluminescent phasor is a new technology for multiplexed, excitation-free imaging at the microscale using luciferase–luciferin pairs. This platform can readily unmix the broad, overlapping emission spectra of bioluminescent reporters, making possible the dynamic tracking of cellular and molecular features over prolonged time periods.
This is a preview of subscription content, access via your institution
Access options
Access Nature and 54 other Nature Portfolio journals
Get Nature+, our best-value online-access subscription
$29.99 /Â 30Â days
cancel any time
Subscribe to this journal
Receive 12 print issues and online access
$259.00 per year
only $21.58 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
References
Suzuki, K. et al. Five colour variants of bright luminescent protein for real-time multicolour bioimaging. Nat. Commun. 7, 13718 (2016). This paper discusses a series of ultrabright reporters for bioluminescence microscopy.
Fereidouni, F., Bader, A. N. & Gerritsen, H. C. Spectral phasor analysis allows rapid and reliable unmixing of fluorescence microscopy spectral images. Opt. Express 20, 12729–12741 (2012). This paper introduces spectral phasor analysis for unmixing fluorescence spectra.
Cutrale, F. et al. Hyperspectral phasor analysis enables multiplexed 5D in vivo imaging. Nat. Methods 14, 149–152 (2017). This paper showcases rapid unmixing of multiple spectrally overlapping fluorophores via spectral phasor analysis.
Dvornikov, A. & Gratton, E. Hyperspectral imaging in highly scattering media by the spectral phasor approach using two filters. Biomed. Opt. Express 9, 3503–3511 (2018). This paper demonstrates a streamlined, inexpensive setup for light collection that is relevant to spectral phasor analysis.
Yeh, H. W. & Ai, H. W. Development and applications of bioluminescent and chemiluminescent reporters and biosensors. Annu. Rev. Anal. Chem. 12, 129–150 (2019). This Review article highlights bioluminescent biosensors, including various BRET-based reporters.
Additional information
Publisher’s note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
This is a summary of: Yao, Z. et al. Multiplexed bioluminescence microscopy via phasor analysis. Nat. Methods https://doi.org/10.1038/s41592-022-01529-9 (2022)
Rights and permissions
About this article
Cite this article
Spectral phasor analysis enables multiplexed microscopy with bioluminescent probes. Nat Methods 19, 801–802 (2022). https://doi.org/10.1038/s41592-022-01530-2
Published:
Issue Date:
DOI: https://doi.org/10.1038/s41592-022-01530-2
