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Transgenic Caenorhabditis elegans expressing green fluorescent protein (GFP) under the control of the C12D8.1 promoter, superimposed on a post-embryonic development chronogram. Vidal and colleagues quantify the spatiotemporal activity of worm promoters using flow cytometry to record longitudinal GFP intensity profiles (p 663). Credit: Eric Smith
FDA left no doubt after a May meeting that it plans to continue pressuring sponsors of erythropoiesis-stimulating agents for more definitive clinical data.
A self-made billionaire and serial entrepreneur of numerous medical device and biotech ventures, Mann has been exemplary in his philanthropy. This has not meant that his Alfred E. Mann Foundation for Biomedical Engineering (AMFBE) has been without its critics.
A group of academics, industry executives and security experts propose an oversight framework to address concerns over the security of research involving commercial DNA synthesis.
Truly comprehensive proteome analysis is highly desirable in systems biology and biomarker discovery efforts. But complete proteome characterization has been hindered by the dynamic range and detection sensitivity of experimental designs, which are not adequate to the very wide range of protein abundances. Experimental designs for comprehensive analytical efforts involve separation followed by mass spectrometry–based identification of digested proteins. Because results are generally reported as a collection of identifications with no information on the fraction of the proteome that was missed, they are difficult to evaluate and potentially misleading. Here we address this problem by taking a holistic view of the experimental design and using computer simulations to estimate the success rate for any given experiment. Our approach demonstrates that simple changes in typical experimental designs can enhance the success rate of proteome analysis by five- to tenfold.