We continue our explanation of the peer review process at Nature Methods.

Organoids are an emerging way to model the fraught complexity of tumors.

After having been largely neglected for more than a century, the tiny marine animal Trichoplax adhaerens is enjoying a revival and is now poised to become a mainstream model organism for the study of epithelial evolution, diversification and specialization.

Kilosort4 is a spike-sorting algorithm with improved performance compared to previous versions, owing to the use of a graph-based clustering approach. The tool extracts the activity of individual neurons from electrophysiological recordings acquired with, for example, Neuropixels electrodes.

Molecular Pixelation is an optics-free method that uses DNA-tagged antibodies to enable identification of the relative location of proteins on single cells.

Combining genome-wide CRISPR–Cas9-mediated base editors with temporally resolved phosphoproteomics enables the functional screening of thousands of post-translational modification sites involved in T cell activation.

ESI-cryoPrep is a cryo-EM specimen preparation method that employs electrospray ionization techniques to deposit charged macromolecule-containing droplets on EM grids. Demonstrated across various protein samples, this approach effectively prevents biomolecule adsorption at air–water or graphene–water interfaces, addressing challenges related to protein denaturation and preferred orientation.

Super-resolution imaging of reference and target structures enables precise determination of the labeling efficiency of high-affinity binding proteins in cells for improved quantitative assessment of protein organization at the single-molecule level.

SABR-IIs are engineered bifunctional receptors that enable CD4⁺ T cell antigen discovery.

Generating training data for training deep-learning-based tools is time consuming. The DELiVR pipeline facilitates this process as demonstrated in this study on detecting c-Fos⁺ cells or microglia in the brain, following tissue clearing and imaging with light-sheet microscopy.

Custom-built mathematical models make the immune response more predictable and offer mechanistic insights into fundamental immunology.

To those who seek transcriptomic information at high resolution, scale and throughput, single-cell RNA sequencing brings the data. Scientists share tips and future plans as they reflect on the method’s rise to stardom.

For a postdoctoral fellowship, it’s advisable to be selective about lab choice and to be clear about expectations.