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The cover photo, the winner of Nikon’s Small World Photomicrography Competition, features a juvenile zebrafish captured using fluorescence labeling, confocal microscopy and image stacking. Bones and scales are labeled in blue, lymphatic vessels in orange.
Algorithms trained to interpret microscope data can greatly extend the information that can be derived from the resulting images, or even optimize how imaging experiments are conducted.
Massively parallel reporter assays (MPRAs) enable high-throughput assessments of regulatory elements in single experiments. This work compares nine MPRA designs and reports how differences in reporter assays influence the results of MPRAs.
CRISPRi activities are measured from dCas9 fusions with 57 KRAB domains, which lead to the identification of ZIM3 KRAB–dCas9 fusion as a superior repressor.
vLUME is a complete virtual reality environment for visualizing, analyzing and interacting with three-dimensional single-molecule localization microscopy data.
An open-source software platform, Genuage, enables the easy perception of, interaction with and analysis of multidimensional point clouds in virtual reality.
Long-read metagenomics offers a valuable approach for profiling bacterial communities. This work presents a long-read assembler, metaFlye, that specifically addresses the challenges of assembling metagenomes.
MSFragger-Glyco allows identification of N- and O-linked glycopeptides using the localization-aware open search strategy of the MSFragger search engine.
O-Pair search identifies O-glycopeptides and localizes O-glycosites using a fragment-ion-indexed open modification search combined with a graph-based approach. It also introduces a classification scheme to unify data reporting for glycoproteomics.
A genetically encoded acetylcholine sensor with improved sensitivity allows detection of cholinergic neurotransmission in vivo in the Drosophila and mouse brain.
Red and yellow versions of the genetically encoded dopamine sensor dLight1 have been developed and allow multiplexed imaging of dopamine with neurotransmitter or cell-type-specific calcium combined with green sensors or actuators, as demonstrated ex vivo and in behaving rodents.
Red and improved green versions of the genetically encoded dopamine sensor GRABDA have been developed. These neurotransmitter sensors are used alone or in combination with, for example, calcium sensors in behaving fruit flies and rodents.