Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain
the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in
Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles
and JavaScript.
Astrocytes generated at physiological oxygen tension from human embryonic stem cellderived neural precursors. Immature astrocytes express vimentin (green), whereas a more mature subpopulation also expresses glial fibrillary acidic protein (GFAP, red). Cell nuclei are stained with DAPI (blue). The protocol describes efficient neural precursor cell, motor neuron and dopaminergic neuron generation at 3% oxygen.