Table 1 List of extracted features.
Feature Name | Description | Category |
|---|---|---|
Diameter-RB | Diameter along the interrogation rainbow. It is insensitive to flow rate fluctuation. For higher accuracy, it is calibrated by the spatial nonuniform distribution of rainbow wavelengths. | Morphology |
Diameter-FL | Diameter along the flow direction. It is sensitive to flow rate fluctuation, but can be a candidate parameter for monitoring flow speed and channel condition. | Morphology |
Tight Area | Total number of pixels in the segmented region in the phase image. | Morphology |
Perimeter | Total number of pixels around the boundary of each segmented region. | Morphology |
Circularity | 4Ï€ (Tight Area)/Perimeter2 | Morphology |
Major Axis | Considering the cell as elliptical in lateral imaging plane, the length of the major axis of the ellipse with a normalized second central moment same as the cell. | Morphology |
Orientation | Angle between the flow direction and the major axis of the cell elliptical shape. | Morphology |
Loose Area | Total number of pixels in the expanded segmented region for measurement of the pixel intensities. | Morphology |
Median Radius | The median distance of any pixel in the object to the closest pixel outside of the object. | Morphology |
OPD-1 | Integrated optical path length difference within the entire segmented area (cell), calibrated by the power distribution within different wavelength components of the incident laser pulses. | Optical Phase |
OPD-2 | Integrated optical path length difference within the entire segmented area (cell). In addition to the calibration of OPD-1, it is calibrated by the pulse-to-pulse fluctuations within a 1µs detection window. | Optical Phase |
Refractive index | The mean refractive index difference between the object and the surrounding liquid (buffer solution), which is calculated based on OPD-2 and size measurement (see detail in Section Methods). Refractive index difference for cells is proportional to their protein concentration. | Optical Phase |
Absorption-1 | Mean absorption coefficient within the entire segmented area (cell). It is calibrated by the power distribution within different wavelength components of the incident laser pulses and by the pulse-to-pulse fluctuations within a 1µs detection window. This parameter corresponds to an absorption-dominant model for the cell. | Optical Loss |
Absorption-2 | Mean absolute absorption coefficient within the entire segmented area (cell). It is calibrated by the power distribution within different wavelength components of the incident laser pulses and by the pulse-to-pulse fluctuations within a 1µs detection window. This parameter corresponds to an absorption-dominant model for the cell. | Optical Loss |
Scattering-1 | Mean optical loss within the entire segmented area (cell). It is calibrated by the power distribution within different wavelength components of the incident laser pulses and by the pulse-to-pulse fluctuations within a 1 detection window. This parameter corresponds to a scattering-dominant model for the cell. | Optical Loss |
Scattering-2 | Mean absolute optical loss within the entire segmented area (cell). It is calibrated by the power distribution within different wavelength components of the incident laser pulses and by the pulse-to-pulse fluctuations within a 1µs detection window. This parameter corresponds to a scattering-dominant model for the cell. | Optical Loss |
