Abstract
A FILM of living bacteria in Agar was distributed over very thin (cover-slip) glass sterilised; this thin glass alone separated the film from the negative. After exposure over a mirror for two hours, and then forty-eight hours' incubation, the bacteria behind the transparent parts of the negative were killed; those behind the opaque parts developed normally; those partly protected were retarded, giving the half tones. The photograph was sharper when first made, several weeks before reproduction.
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Photograph of a Landscape in Living and Dead Bacteria. Nature 50, 250 (1894). https://doi.org/10.1038/050250a0
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DOI: https://doi.org/10.1038/050250a0