Abstract
A20 is a Cys2/Cys2 zinc finger protein which is induced by a variety of inflammatory stimuli and which has been characterized as an inhibitor of cell death by a yet unknown mechanism. In order to clarify its molecular mechanism of action, we used the yeast two-hybrid system to screen for proteins that interact with A20. A cDNA fragment was isolated which encoded a portion of a novel protein (TXBP151), which was recently found to be a human T-cell leukemia virus type-I (HTLV-I) Tax-binding protein. The full-length 2386 bp TXBP151 mRNA encodes a protein of 86 kDa. Like A20, overexpression of TXBP151 could inhibit apoptosis induced by tumour necrosis factor (TNF) in NIH3T3 cells. Moreover, transfection of antisense TXBP151 partially abolished the anti-apoptotic effect of A20. Furthermore, apoptosis induced by TNF or CD95 (Fas/APO-1) was associated with proteolysis of TXBP151. This degradation could be inhibited by the broad-spectrum caspase inhibitor zVAD-fmk or by expression of the cowpox virus-derived inhibitor CrmA, suggesting that TXBP151 is a novel substrate for caspase family members. TXBP151 was indeed found to be specifically cleaved in vitro by members of the caspase-3-like subfamily, viz. caspase-3, caspase-6 and caspase-7. Thus TXBP151 appears to be a novel A20-binding protein which might mediate the anti-apoptotic activity of A20, and which can be processed by specific caspases.
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Alnemri ES, Livingston DJ, Nicholson DW, Salvesen G, Thornberry NA, Wong WW and Yuan J. . 1996 Cell 87: 171.
Cheng EH, Kirsch DG, Clem RJ, Ravi R, Kastan MB, Bedi A, Ueno K and Hardwick JM. . 1997 Science 278: 1966–1968.
Chinnayan AM, Tepper CG, Seldin MF, O'Rourke K, Kischkel FC, Hellbardt S and Dixit VM. . 1996 J. Biol. Chem. 271: 4961–4965.
Cooper JT, Stroka DM, Brostjan C, Palmetshofer A, Bach FH and Ferran C. . 1996 J. Biol. Chem. 271: 18068–18073.
De Valck D, Heyninck K, Van Criekinge W, Contreras R, Beyaert R and Fiers W. . 1996 FEBS Lett. 384: 61–64.
De Valck D, Heyninck K, Van Criekinge W, Vandenabeele P, Fiers W and Beyaert R. . 1997 Biochem. Biophys. Res. Commun. 238: 590–594.
Dixit VM, Green S, Sarma V, Holzman LB, Wolf FW, O'Rourke K, Ward PA, Prochownik EV and Marks RM. . 1990 J. Biol. Chem. 265: 2973–2978.
Fearnhead HO, Dinsdale D and Cohen GM. . 1995 FEBS Lett. 375: 283–288.
Fujita N, Nagahashi A, Nagashima K, Rokudai S and Tsuruo T. . 1998 Oncogene 17: 1295–1304.
Gachon F, Peleraux A, Thebault S, Dick J, Lemasson I, Devaux C and Mesnard JM. . 1998 J. Virol. 72: 8332–8337.
Heyninck K and Beyaert R. . 1999 FEBS Lett. 442: 147–150.
Hennet T, Bertoni G, Richter C and Peterhans E. . 1993 Cancer Res. 53: 1456–1460.
Jäättelä M, Mouritzen H, Elling F and Bastholm L. . 1996 J. Immunol. 156: 1166–1173.
Jänicke RU, Lee FHH and Porter AG. . 1994 Mol. Cell Biol. 14: 5661–5670.
Jin D-Y, Teramoto H, Giam C-Z, Chun RF, Gutkind JS and Jeang K-T. . 1997 J. Biol. Chem. 272: 25816–25823.
Jin D-Y and Jeang K-T. . 1997 Nucl. Acids Res. 25: 379–387.
Jin D-Y, Spencer F and Jeang K-T. . 1998 Cell 93: 81–91.
Korioth F, Gieffers C, Maul GG and Frey J. . 1995 J. Cell. Biol. 130: 1–13.
Laherty CD, Hu HM, Opipari AW, Wang F and Dixit VM. . 1992 J. Biol. Chem. 267: 24157–24160.
Laherty CD, Perkins ND and Dixit VM. . 1993 J. Biol. Chem. 268: 5032–5039.
Liston P, Roy N, Tamai K, Lefebvre C, Baird S, Cherton-Horvat G, Farahani R, McLean M, Ikeda JE, MacKenzie A and Korneluk RG. . 1996 Nature 379: 349–353.
Los M, Van de Craen M, Penning LC, Schenk H, Westendorp M, Baeuerle PA, Dröge W, Krammer PH, Fiers W and Schulze-Osthoff K. . 1995 Nature 375: 81–83.
Majone F, Semmes OJ and Jeang K-T. . 1993 Virology 193: 456–459.
Mertens N, Remaut E and Fiers W. . 1995 Gene 164: 9–15.
Muslin AJ, Tanner JW, Allen PM and Shaw AS. . 1996 Cell 84: 889–897.
Natoli G, Costanzo A, Guido F, Moretti F, Bernardo A, Burgio VL, Agresti C and Levrero M. . 1998 J. Biol. Chem. 273: 31262–31272.
Nicholson DW and Thornberry NA. . 1997 Trends Biochem. Sci. 22: 299–306.
O'Mahoney JV and Adams TE. . 1994 DNA Cell Biol. 13: 1227–1232.
Opipari AW, Boguski MS and Dixit VM. . 1990 J. Biol. Chem. 265: 14705–14708.
Opipari AW, Hu HM, Yabkowitz R and Dixit VM. . 1992 J. Biol. Chem. 267: 12424–12427.
Porter AG, Ng P and Jänicke RU. . 1997 BioEssays 19: 501–507.
Pronk GJ, Ramer K, Amiri P and Williams LT. . 1996 Science 271: 808–810.
Rosin O, Koch C, Radant I, Schmitt I, Semmes OJ, Jeang K-T and Grassman R. . 1998 J. Biol. Chem. 273: 6698–6703.
Rubin BY, Smith LJ, Hellermann GR, Lunn RM, Richardson NK and Anderson SL. . 1988 Cancer Res. 48: 6006–6010.
Sarma V, Lin Z, Clark L, Rust BM, Tewari M, Noelle RJ and Dixit VM. . 1995 J. Biol. Chem. 270: 12343–12346.
Schotte P, Van Criekinge W, Van de Craen M, Van Loo G, Desmedt M, Grooten J, Cornelissen M, De Ridder L, Vandekerckhove J, Fiers W, Vandenabeele P and Beyaert R. . 1998 Biochem. Biophys. Res. Commun. 251: 379–387.
Song HY, Rothe M and Goeddel DV. . 1996 Proc. Natl. Acad. Sci. USA 93: 6721–6725.
Sporn SA, Eierman DF, Johnson CE, Morris J, Martin G, Ladner M and Haskill S. . 1990 J. Immunol. 144: 4434–4441.
Tewari M and Dixit VM. . 1995 J. Biol. Chem. 270: 3255–3260.
Thornberry NA, Ranon TA, Pieterson EP, Rasper DM, Timkey T, Garciacalvo M, Houtzager VM, Nordstrom PA, Roy S, Vaillancourt JP, Chapman KT and Nicholson DW. . 1997 J. Biol. Chem. 272: 17907–17911.
Thornberry NA and Lazebnik Y. . 1998 Science 281: 1312–1316.
Van de Craen M, Vandenabeele P, Declercq W, Van den Brande I, Van Loo G, Molemans F, Schotte P, Van Criekinge W, Beyaert R and Fiers W. . 1997 FEBS Lett. 403: 61–69.
Vincenz C and Dixit VM. . 1996 J. Biol. Chem. 271: 20029–20034.
Widmann C, Gibson S and Johnson GL. . 1998 J. Biol. Chem. 273: 7141–7147.
Zen K, Karsan A, Eunson T, Yee E, and Harlan JM. . 1998 Exp. Cell Res. 243: 425–433.
Zhou Q, Snipas S., Orth K, Muzio M, Dixit VM and Salvesen G. . 1997 J. Biol. Chem. 272: 7797–7800.
Acknowledgements
The authors thank S Dewaele for construction of a L929r2 cDNA library, and R Cocquyt and F Molemans for DNA sequencing. We also thank A Meeus and M Van den Hemel for their assistance in performing transient transfections. I Van den Brande and Y Bruynooghe are acknowledged for purification of caspases and technical assistance in performing in vitro caspase assays. Rudi Beyaert is a postdoctoral research assistant with the Fonds voor Wetenschappelijk Onderzoek-Vlaanderen. Research was supported by the Interuniversitaire Attractiepolen, an EC BIOMED2 grant and an EC-TMR grant.
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De Valck, D., Jin, DY., Heyninck, K. et al. The zinc finger protein A20 interacts with a novel anti-apoptotic protein which is cleaved by specific caspases. Oncogene 18, 4182–4190 (1999). https://doi.org/10.1038/sj.onc.1202787
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DOI: https://doi.org/10.1038/sj.onc.1202787
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