Figure 6 | Oncogene

Figure 6

From: Inhibition of tumor growth and metastasis of human melanoma by intracellular anti-ATF-1 single chain Fv fragment

Figure 6

Intracellular expression of ScFv anti-ATF-1 inhibits Tg-induced CRE-dependent promoter activation. CAT activity was increased in MeWo-P and Neo-transfected cells by 15–20-fold following treatment with 1 μM Tg 24 h (compare lane 7 to lanes 2, 3, and 4). However, CAT activity was induced only by sixfold and threefold in MeWo-ScFv-P and ScFv-C4, respectively (lanes 5 and 6). Lane 1, Basic, control plasmid without the CRE-dependent promoter. -Fold activation is calculated in comparison to untreated cells (lane 7), which was given the reference value of 1. Figure is representative of three experiments performed

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