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  • Oncogenomics
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Gene expression profiles of pancreatic cancer and stromal desmoplasia

Oncogene volume 20, pages 7437–7446 (2001)Cite this article

Abstract

Gene expression studies were undertaken in normal pancreas and pancreatic adenocarcinomas to determine new candidate genes that can potentially be used as markers of the disease. The characteristic desmoplastic stromal reaction of pancreatic adenocarcinoma greatly hampers expression studies in this tumour type, and usually necessitates time-consuming tissue microdissection for enrichment of the tumour cell population. We show that fine needle aspiration of cancer provides a fast and efficient way of obtaining samples highly enriched in tumour cells with sufficient yields of RNA. Using Atlas cancer cDNA arrays with 588 cancer-related genes, we describe gene expression profiles of normal pancreas, bulk pancreatic tumour tissues and pancreatic tumour aspirates containing more than 95% tumour cells. Analysis of bulk tissue specimens revealed differentially expressed genes belonging predominantly to the stromal component of the tumour. This contrasted with the results obtained from tumour-cell enriched samples. Several genes already described in pancreatic cancer (caspase 8, TIMP1, CD9, IL-13) were also differentially expressed in our study. Furthermore, we found dysregulated expression of genes not previously associated with pancreatic adenocarcinoma, such as Rac 1, GLG1, NEDD5, RPL-13a, RPS9 and members of the Wnt5A gene family. In summary, we present a panel of genes newly identified in the pathogenesis of pancreatic adenocarcinoma and demonstrate that fine needle aspirates of the tumour mass are a convenient source of material for gene expression studies in tumours accompanied by desmoplastic reactions.

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Acknowledgements

Supported by Imperial Cancer Research Fund; Special Trustees of Hammersmith Hospital; Associazione Italiana Ricerca sul Cancro (AIRC) to A Scarpa, Milan, Italy; Consorzio Studi Universitari di Verona, Italy; Ministero Università e Ricerca Scientifica e Tecnologica (MURST), Cofin MM06158571 – Cofin 9906195987, Rome, Italy.

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Author notes

  1. Tatjana Crnogorac-Jurcevic and Evangelis Efthimiou: T Crnogorac-Jurcevic and E Efthimiou contributed equally to this work

Authors and Affiliations

  1. Imperial Cancer Research Fund Molecular Oncology Unit, Imperial College School of Medicine at Hammersmith Campus, London, UK

    Tatjana Crnogorac-Jurcevic, Evangelis Efthimiou, Ekaterina Blaveri, Benoit Terris, Melanie Jones & Nicholas R Lemoine

  2. Oxford Glycosciences Proteome Discovery, Abingdon, UK

    Kerry Tyson

  3. Department of Pathology, Università di Verona, Italy

    Paola Capelli, Antonella Baron & Aldo Scarpa

  4. Department of Surgery, Università di Verona, Italy

    Claudio Bassi

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  1. Tatjana Crnogorac-Jurcevic
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  2. Evangelis Efthimiou
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  3. Paola Capelli
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  11. Nicholas R Lemoine
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Correspondence to Nicholas R Lemoine.

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Crnogorac-Jurcevic, T., Efthimiou, E., Capelli, P. et al. Gene expression profiles of pancreatic cancer and stromal desmoplasia. Oncogene 20, 7437–7446 (2001). https://doi.org/10.1038/sj.onc.1204935

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