Figure 5
From: Promoter-specific p53-dependent histone acetylation following DNA damage
Mutation of p53 phosphorylation sites. Lentiviral vectors were used to express wild-type p53 and phosphomutant p53 in p53 knockout HCT116 cells. The N6A mutant contains alanine substitutions of six phosphorylation sites at the p53 N-terminus. ChIP was performed from cells treated with 375 μ M 5FU for 9 h. (a) Western blot for p53, p21, and MDM2. (b) ChIP for H4 acetylation. (c) ChIP for H3 acetylation
