Abstract
Identifying changes in DNA copy number can pinpoint genes that may be involved in tumor development. Here we have defined the smallest overlapping regions of imbalance (SORI) in testicular germ cell tumors other than the 12p region, which has been previously investigated. Definition of the regions was achieved through comparative genomic hybridization (CGH) analysis of a 4559 cDNA clone microarray. A total of 14 SORI were identified, which involved at least five of the 11 samples analysed. Many of these refined regions were previously reported using chromosomal or allelic imbalance studies. The SORI included gain of material from the regions 4q12, 17q21.3, 22q11.23 and Xq22, and loss from 5q33, 11q12.1, 16q22.3 and 22q11. Comparison with parallel chromosomal CGH data supported involvement of most regions. The various SORI span between one and 20 genes and highlight potential oncogenes/tumor suppressor genes to be investigated further (Supplementary material is available at http://www.crukdmf.icr.ac.uk/array/array.html).
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References
Bartkova J, Falck J, Rajpert-De Meyts E, Skakkebaek NE, Lukas J and Bartek J . (2001). Oncogene, 20, 5897–5902.
Bokemeyer C, Kuczyk MA, Dunn T, Serth J, Hartmann K, Jonasson J, Pietsch T, Jonas U and Schmoll HJ . (1996). J. Cancer Res. Clin. Oncol., 122, 301–306.
Bosl GJ and Motzer RJ . (1997). N. Engl. J. Med., 337, 242–253.
Chen J, Liu B, Liu Y, Han Y, Yu H, Zhang Y, Lu L, Zhen Y and Hui R . (2002). Biochem. Biophys. Res. Commun., 294, 161–166.
Clark J, Edwards S, John M, Flohr P, Gordon T, Maillard K, Giddings I, Brown C, Bagherzadeh A, Campbell C, Shipley J, Wooster R and Cooper CS . (2002). Genes Chromosomes Cancer, 34, 104–114.
Draper JS, Smith K, Gokhale P, Moore HD, Maltby E, Johnson J, Meisner L, Zwaka TP, Thomson JA and Andrews PW . (2004). Nat. Biotechnol., 22, 53–54.
Huang Z, Traugh JA and Bishop JM . (2004). Mol. Cell. Biol., 24, 1582–1594.
Izquierdo MA, Van der Valk P, Van Ark-Otte J, Rubio G, Germa-Lluch JR, Ueda R, Scheper RJ, Takahashi T and Giaccone G . (1995). J. Pathol., 177, 253–258.
Jakobi R, McCarthy CC, Koeppel MA and Stringer DK . (2003). J. Biol. Chem., 278, 38675–38685.
Karan D, Kelly DL, Rizzino A, Lin MF and Batra SK . (2002). Carcinogenesis, 23, 967–975.
Kemmer K, Corless CL, Fletcher JA, McGreevey L, Haley A, Griffith D, Cummings OW, Wait C, Town A and Heinrich MC . (2004). Am. J. Pathol., 164, 305–313.
Korn WM, Oide Weghuis DE, Suijkerbuijk RF, Schmidt U, Otto T, du Manoir S, Geurts van Kessel A, Harstrick A, Seeber S and Becher R . (1996). Genes Chromosomes Cancer, 17, 78–87.
Kraggerud SM, Skotheim RI, Szymanska J, Eknaes M, Fossa SD, Stenwig AE, Peltomaki P and Lothe RA . (2002). Genes Chromosomes Cancer, 34, 168–174.
Looijenga LH, de Leeuw H, van Oorschot M, van Gurp RJ, Stoop H, Gillis AJ, de Gouveia Brazao CA, Weber RF, Kirkels WJ, van Dijk T, von Lindern M, Valk P, Lajos G, Olah E, Nesland JM, Fossa SD and Oosterhuis JW . (2003). Cancer Res., 63, 7674–7678.
Looijenga LH, Rosenberg C, van Gurp RJ, Geelen E, van Echten-Arends J, de Jong B, Mostert M and Wolter Oosterhuis J . (2000). J. Pathol., 191, 187–192.
Lu YJ, Dong XY, Shipley J, Zhang RG and Cheng SJ . (1999). Lung Cancer, 23, 61–66.
Mitchell RA and Bucala R . (2000). Semin. Cancer Biol., 10, 359–366.
Mostert MM, van de Pol M, Olde Weghuis D, Suijkerbuijk RF, Geurts van Kessel A, van Echten J, Oosterhuis JW and Looijenga LH . (1996). Cancer Genet Cytogenet., 89, 146–152.
Mostofi FK . (1973). Cancer, 32, 1186–1201.
Ottesen AM, Kirchhoff M, De-Meyts ER, Maahr J, Gerdes T, Rose H, Lundsteen C, Petersen PM, Philip J and Skakkebaek NE . (1997). Genes Chromosomes Cancer, 20, 412–418.
Pollack JR, Perou CM, Alizadeh AA, Eisen MB, Pergamenschikov A, Williams CF, Jeffrey SS, Botstein D and Brown PO . (1999). Nat. Genet., 23, 41–46.
Rapley EA, Crockford GP, Easton DF, Stratton MR and Bishop DT . (2003). Apmis, 111, 128–133 ; discussion 33–35.
Rapley EA, Hockley S, Warren W, Johnson L, Huddart R, Crockford G, Forman D, Leahy MG, Oliver DT, Tucker K, Friedlander M, Phillips KA, Hogg D, Jewett MA, Lohynska R, Daugaard G, Richard S, Heidenreich A, Geczi L, Bodrogi I, Olah E, Ormiston WJ, Daly PA, Looijenga LH, Guilford P, Aass N, Fossa SD, Heimdal K, Tjulandin SA, Liubchenko L, Stoll H, Weber W, Einhorn L, Weber BL, McMaster M, Greene MH, Bishop DT, Easton D and Stratton MR . (2004). Br. J. Cancer, 90, 2397–2401.
Ren Y, Chan HM, Li Z, Lin C, Nicholls J, Chen CF, Lee PY, Lui V, Bacher M and Tam PK . (2004). Oncogene., 23, 4146–4154.
Reome JB, Hylind JC, Dutton RW and Dobrzanski MJ . (2004). Clin. Immunol., 111, 69–81.
Rodriguez S, Jafer O, Goker H, Summersgill BM, Zafarana G, Gillis AJ, van Gurp RJ, Oosterhuis JW, Lu YJ, Huddart R, Cooper CS, Clark J, Looijenga LH and Shipley JM . (2003). Oncogene, 22, 1880–1891.
Sambrook J and Russell W . (2001). Molecular Cloning, A Laboratory Manual, Vol. 3. New York: Cold Spring Harbor Laboratory Press.
Sandberg AA, Meloni AM and Suijkerbuijk RF . (1996). J. Urol., 155, 1531–1556.
Shuin T, Misaki H, Kubota Y, Yao M and Hosaka M . (1994). Cancer, 73, 1721–1727.
Skotheim RI, Diep CB, Kraggerud SM, Jakobsen KS and Lothe RA . (2001a). Cancer Genet. Cytogenet., 127, 64–70.
Skotheim RI, Kraggerud SM, Fossa SD, Stenwig AE, Gedde-Dahl Jr T, Danielsen HE, Jakobsen KS and Lothe RA . (2001b). Neoplasia, 3, 196–203.
Skotheim RI, Monni O, Mousses S, Fossa SD, Kallioniemi OP, Lothe RA and Kallioniemi A . (2002). Cancer Res., 62, 2359–2364.
Summersgill BM, Jafer O, Wang R, Goker H, Niculescu-Duvaz I, Huddart R and Shipley J . (2001). Cancer Genet. Cytogenet., 128, 120–129.
van Echten J, Oosterhuis JW, Looijenga LH, van de Pol M, Wiersema J, te Meerman GJ, Schaffordt Koops H, Sleijfer DT and de Jong B . (1995). Genes Chromosomes Cancer, 14, 133–144.
Watson JV, Stewart J, Evan GI, Ritson A and Sikora K . (1986). Br. J. Cancer, 53, 331–337.
Zafarana G, Grygalewicz B, Gillis AJ, Vissers LE, van de Vliet W, van Gurp RJ, Stoop H, Debiec-Rychter M, Oosterhuis JW, van Kessel AG, Schoenmakers EF, Looijenga LH and Veltman JA . (2003). Oncogene, 22, 7695–7701.
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We thank the Lance Armstrong Foundation, Cancer Research UK and the Dutch Cancer Society (including Grant DDHK 97-1685) for their support.
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McIntyre, A., Summersgill, B., Jafer, O. et al. Defining minimum genomic regions of imbalance involved in testicular germ cell tumors of adolescents and adults through genome wide microarray analysis of cDNA clones. Oncogene 23, 9142–9147 (2004). https://doi.org/10.1038/sj.onc.1208115
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DOI: https://doi.org/10.1038/sj.onc.1208115
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