Table 3 Summary of genomic PCR, RT-PCR and MSP analysis

OSCC cell lines														Other SCC cell lines
Genomic PCR
	HSC2	HSC3	HSC4	OSC4	OSC5	OSC6	SCC9	SCC25	Ca9–22	SAS	HO1-U1	KB	KN	TE4	TE8	EBC1	ME180
Exon 1									HD	HD			2/13 (15%)
Exon 2									HD	HD			2/13 (15%)
Exon 3									HD				1/13 (7%)
Exon 4									HD	HD	HD		3/13 (23%)
*RT-PCR*
	*HSC2*	*HSC3*	*HSC4*	*OSC4*	*OSC5*	*OSC6*	*SCC9*	*SCC25*	*Ca9–22*	*SAS*	*HO1-U1*	KB	KN	*TE4*	*TE8*	*EBC1*	*ME180*
Exon 2	NE								NE	NE			3/13 (23%)	NE
Exon 3									NE	NE			2/13 (15%)	NE
Exon 3–4									NE	NE			2/13 (15%)	NE
*Methylation-specific PCR* (+: *methylated* , −: *unmethylated*)
*HSC2*	*HSC3*	*HSC4*	*OSC4*	*OSC5*	*OSC6*	*SCC9*	*SCC25*	*Ca9–22*	*SAS*	*HO1-U1*	KB	KN	*TE4*	*TE8*	*EBC1*	*ME180*
+	−	−	−	−	−	−	−	−	+	+	−	−	+	−	−	−
*OSCC primary tumors*
	T1	T2	T3	T4	T5	T6	T7	T8	T9	*T10*
Exon 1		HD		HD	HD			HD		HD
Exon 2	HD						HD
Exon 3					HD	HD
Exon 4				HD
	*T11*	*T12*	*T13*	*T14*	*T15*	*T16*	*T17*	*T18*	*T19*	*T20*
Exon 1		HD					HD	HD	HD		9/20 (45%)
Exon 2	HD	HD									4/20 (20%)
Exon 3											2/20 (10%)
Exon 4	HD	HD	HD	HD	HD	HD					7/20 (35%)

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