Abstract
IN his letter appearing under the above title1, Dr. F. C. Happold criticizes the procedure used by Dr. E. C. Barton-Wright and Mr. R. G. Booth2 in the determination of riboflavin, and insists on the importance of calcium in the nutrient medium and mentions the work of Chattaway, Happold and Sandford3. The evidence in the latter paper to which he evidently refers consists in an experiment in which a series of increasing doses of calcium chloride was added to the nutrient medium. The authors do not seem to have appreciated fully a limiting factor in the very low solubility of calcium in the presence of excess of phosphate at pH 6.6–6.8. The solubility product of tricalcium phosphate is about 1032, but may be perhaps 1031 in the presence of the other minerals contained in Chattaway, Happold and Sandford's nutrient medium4,5.
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References
Happold, F. C., NATURE, 152, 414 (1943).
Barton-Wright, E. C., and Booth, R. G., Biochem. J., 37, 25 (1943).
Chattaway, F. W., Happold, F. C., and Sandford, Mary, Biochem. J., 37, 298 (1943).
Holt, E., La Mer, V. K., and Chown, H. B., J. Biol. Chem., 64, 509 (1925).
Hopkins, R. H., and Amphlett, P. H., J. Inst. Brew., 45, 365 (1939).
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HOPKINS, R. Microbiological Assay of Riboflavin. Nature 152, 724–725 (1943). https://doi.org/10.1038/152724b0
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DOI: https://doi.org/10.1038/152724b0
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Microbiological Assay of Riboflavine
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