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Cord Blood Stem Cells

CD34+ progenitors are reproducibly recovered in thawed umbilical grafts, and positively influence haematopoietic reconstitution after transplantation

Bone Marrow Transplantation volume 39, pages 453–460 (2007)Cite this article

Abstract

Cord blood (CB) units are increasingly used for allogeneic transplantation. Cell dose, a major factor for CB selection, is evaluated before freezing by each CB bank, using various techniques. This may introduce variability and affect the prediction of cell recovery after thawing, or haematopoietic reconstitution. Forty-two children were transplanted at the same institution with unrelated CB units. All units were thawed and evaluated at the same cell therapy facility, using standard procedures. We investigated: (i) factors that affect cell loss after thawing, and (ii) the importance of CD34+ cell doses. Prefreeze and post-thaw CD34+ cell doses were statistically correlated, thus suggesting that variability in numeration techniques used by different CB banks does not compromise the biological and clinical value of these figures. CD34+ cell recovery appeared to be correlated with the absolute number of CD34+ cells per frozen bag. Infused CD34+ is the cell dose that better correlates with platelet reconstitution delay; in addition, when using a quartile comparison, haematopoietic recovery appeared to be related with prefreeze and post-thaw CD34+ cell doses. We conclude that enumeration of CD34+ cells in CB units is of biological significance, and may help select CB units and identify patients at risk of delayed recovery.

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Acknowledgements

We thank V Rocha and F Garnier from Eurocord; D Reviron and C Picard for CB selection and HLA typing; I Sielleur for flow cytometry analysis and D. Sandragne for access to patient databases. This work was supported in part by Institut Paoli-Calmettes, and by a grant from the EEC to CC (Eurocord III Program). This work was presented in part at the 2006 EBMT Annual meeting in Hamburg, Germany, and at the 2006 ISCT Annual meeting, in Berlin, Germany.

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Authors and Affiliations

  1. Département de Biologie, Centre de Thérapie Cellulaire et Génique, Institut Paoli-Calmettes, Marseille, France

    C Lemarie, B Calmels & C Chabannon

  2. Réseau Français du Sang Placentaire, France

    C Lemarie, B Calmels, B Dazey, V Lapierre, D Rea & C Chabannon

  3. Centre d'Investigations Cliniques en Biothérapies, Institut Paoli-Calmettes, INSERM, Université de la Méditerranée, Marseille, France

    C Lemarie, B Calmels, C Chabannon & G Michel

  4. Département de Biostatistiques, Institut Paoli-Calmettes, Marseille, France

    B Esterni

  5. Etablissement Français du Sang Aquitaine-Limousin, Bordeaux, France

    B Dazey

  6. Etablissement Français du Sang Bourgogne-Franche Comté, Besançon, France

    V Lapierre

  7. Cord Blood Bank Medicina Trasfusionale, Terapia Cellulare e Criobiologia, Milano, Italy

    L Lecchi

  8. Transplantation Immunology and Stem Cell Bank, Heinrich-Heine-University, Medical Center, Düsseldorf, Germany

    A Meyer

  9. Unité de Thérapie Cellulaire, Hôpital Saint-Louis, Assistance Publique-Höpitaux de Paris, Paris, France

    D Rea

  10. Service d'Hématologie Pédiatrique, Centre Hospitalier Universitaire Timone-Enfants, Assistance Publique-Höpitaux de Marseille, Marseille, France

    I Thuret, H Chambost, C Curtillet & G Michel

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  1. C Lemarie
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Correspondence to C Lemarie.

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Lemarie, C., Esterni, B., Calmels, B. et al. CD34+ progenitors are reproducibly recovered in thawed umbilical grafts, and positively influence haematopoietic reconstitution after transplantation. Bone Marrow Transplant 39, 453–460 (2007). https://doi.org/10.1038/sj.bmt.1705618

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