Abstract
AT the present time there is considerable demand for a suitable synthetic substrate for the routine assay of β-glucuronidase. The most common assay method employs phenolphthalein glucuronide isolated from rabbit urine1. However, the preparation of 4-methylumbelliferone glucuronide from rabbit urine and its use in the fluorimetric assay of β-glucuronidase have been recently described2. This substrate promises certain advantages over phenolphthalein glucuronide, and it is easy to synthesize by the method previously employed for (−)-menthol3 and phenol4 β-D-glucuronides.
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References
di Somma, A. A., J. Biol. Chem., 133, 277 (1940).
Mead, J. A. R., Smith, J. N., and Williams, R. T., Biochem. J., 61, 569 (1955).
Marsh, C. A., J. Chem. Soc., 1578 (1952).
Tsou, K.-C., and Seligman, A. M., J. Amer. Chem. Soc., 75, 1042 (1953).
Robinson, D., and Williams, R. T., Biochem. J., 61, v (1955).
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MARSH, C., LEVVY, G. Synthesis Of 4-Methylumbelliferone β-D-Glucuronide, a Substrate for the Fluorimetric Assay of β-Glucuronidase. Nature 178, 589–590 (1956). https://doi.org/10.1038/178589b0
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DOI: https://doi.org/10.1038/178589b0
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