Differential Analysis by Various Staining Techniques of Structures present in Developing Spermatids of the Silkworm

Abstract

IN the course of an electron microscope investigation¹ of spermiogenesis in the silkworm, Bombyx mori Linné, the formation of a tubular structure and two bands have been detected in the developing spermatids fixed with potassium permanganate or osmium tetroxide, which have not, to our knowledge, been reported previously from light microscopic investigations^2–7. The two bands, which run parallel with the developing spermatid nuclei, were found to be of equal density when the material was fixed with osmium tetroxide, while one of them was less dense when the material was fixed with potassium permanganate.