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Determination of Dipeptidase Activities

Abstract

INVESTIGATIONS of intestinal mucosal dipeptidases have been hampered in the past by the lack of suitable assay methods. The methods in use at present1 require relatively large amounts of substrate and are inadequate for the examination of dipeptidase activities in crude tissue extracts or partially purified preparations. The new assay method described here has a wide application and is simple and rapid. It is convenient even when a large number of determinations is required.

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References

  1. Smith, E. L., in Methods in Enzymology, edit. by Colowick, S. P., and Kaplan, N. O., 2, 93 (Academic Press, New York, 1955).

    Book  Google Scholar 

  2. Saidel, L. J., Goldfarb, A. R., and Waldmann, S., J. Biol. Chem., 197, 285 (1952).

    CAS  PubMed  Google Scholar 

  3. Moore, S., and Stein, W. H., J. Biol. Chem., 211, 907 (1954).

    CAS  PubMed  Google Scholar 

  4. Grassmann, W., and Heyde, W., Z. physiol. Chem., 183, 32 (1929).

    Article  CAS  Google Scholar 

  5. Schmitt, A., and Siebert, G., Biochem. Z., 334, 96 (1961).

    CAS  PubMed  Google Scholar 

  6. Tombs, M. P., Souter, F., and Maclagan, N. F., Biochem. J., 73, 167 (1959).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

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JOSEFSSON, L. Determination of Dipeptidase Activities. Nature 204, 783–784 (1964). https://doi.org/10.1038/204783a0

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  • DOI: https://doi.org/10.1038/204783a0

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