Abstract
IT has recently been shown1,2 that the rabbit excretes 17α-oestradiol as the 3-glucuronoside-17a-N-acetylglu-cosaminide. To the best of our knowledge this is the first N-acetylglucosaminide of a non-sugar to be found in animals, although β-N-acetylglucosaminidase is widely distributed in animal tissues3. It was the object of the work recorded here to determine whether the steroid N-acetylglucosaminide was effectively hydrolysed by the tissue enzyme in rabbits, and whether the enzyme distribution, when measured with this naturally occurring substrate, was the same as that found by previous investigators3,4 who used synthetic substrates. We also wished to compare the relative ability of different tissues to remove the glucuronic acid and the N-acetylglucosamine from the oestradiol molecule.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Layne, D. S., Sheth, N. A., and Kirdani, R. Y., J. Biol. Chem., 239, 3221 (1964).
Layne, D. S., Endocrinology, 76, 600 (1965).
Conchie, J., Findlay, J., and Levvy, G. A., Biochem. J., 71, 318 (1959).
Woolen, J. W., Walker, P. G., and Heyworth, R., Biochem. J., 79, 294 (1961).
Walker, P. G., Woolen, J. W., and Heyworth, R., Biochem. J., 79, 288 (1961).
Levvy, G. A., and Marsh, C. A., Adv. Carbohyd. Chem., 14, 381 (1959).
Wakabayashi, M., and Fishman, W. H., J. Biol. Chem., 236, 996 (1961).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
WHITTEMORE, K., LAYNE, D. Hydrolysis of Two 17α-Oestradiol Glycosides by Rabbit Tissue Glycosidases. Nature 208, 288–289 (1965). https://doi.org/10.1038/208288a0
Issue Date:
DOI: https://doi.org/10.1038/208288a0
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.