Abstract
EBASHI discovered that EGTA or Ca++ insensitive, non-relaxing actomyosin could be made EGTA sensitive by a third protein which exhibited physico-chemical properties characteristic of Bailey's tropomyosin B (ref. 1). However, he found no EGTA-sensitizing activity in Bailey's tropomyosin, and his new protein lost its activity when subjected to Bailey's procedures required for purification. Ebashi concluded that his EGTA-sensitizing protein was a new ‘tropomyosin-like’ protein or a native form of tropomyosin B. He offered no explanation concerning the difference between his ‘tropomyosin-like’ EGTA-sensitizing protein and Bailey's tropomyosin B.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Ebashi, S., and Ebashi, F., J. Biochem., 55, 604 (1964).
Kominz, D. R., Hough, A., Symmonds, P., and Laki, K., Arch. Biochem. Biophys., 50, 148 (1954).
Kominz, D. R., Saad, F., Gladner, J. A., and Laki, K., Arch. Biochem. Biophys., 70, 16 (1957).
Drabikowski, W., and Nowak, E., Acta Biochim. Polon., 12, 61 (1965).
Bailey, K., Biochem. J., 43, 271 (1948).
Cleland, W. W., Biochemistry, 3, 480 (1964).
Boyer, P. D., J. Amer. Chem. Soc., 76, 4331 (1954).
Katz, A. M., Fed. Proc., 24, 208 (1965).
Mueller, H., Fed. Proc., 24, 599 (1965).
Azuma, N., and Watanabe, S., J. Biol. Chem. (in the press).
Azuma, N., and Watanabe, S., J. Biol. Chem. (in the press).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
MUELLER, H. Effect of Bailey's Tropomyosin Purification on EGTA-sensitizing Activity. Nature 209, 1128–1129 (1966). https://doi.org/10.1038/2091128a0
Issue Date:
DOI: https://doi.org/10.1038/2091128a0
This article is cited by
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.