Abstract
SINCE Eddy and Williamson1 showed that the gut juice of the snail Helix pomatia could be used in isolating protoplasts from certain strains of Saccharomyces carlsbergensis and Sacch. cerevisiae, several attempts have been made to isolate protoplasts from yeasts or fungi, using various enzyme preparations2–14. In general, protoplasts or spheroplasts of the susceptible strains were obtained from young culture and at very dilute cell suspensions. Recently, Duell, Inoue and Utter15 improved the method of isolation of the spheroplasts of the yeast, Saccharomyces cerevisiae, by pretreating the yeast cells with 2-mercaptoethylamine and ethylenediaminetetraacetate, followed by treatment with an enzyme from snail gut juice.
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References
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SUGAWARA, S. Spheroplasts from Candida albicans. Nature 212, 92–93 (1966). https://doi.org/10.1038/212092b0
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DOI: https://doi.org/10.1038/212092b0
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