Interchangeability of Ribosomes and Protein Synthesizing Enzymes from Penicillium cyclopium and those prepared from Other Organisms

Abstract

IT has been established that the incorporation of amino-acids into peptide chains in vitro requires the presence of ribosomes, soluble ribonucleic acid (sRNA), soluble enzymes (105,000g supernatant enzymes) and messenger ribonucleic acid. The number of reports on the interchangeability of ribosomes prepared from one organism and soluble enzymes prepared from another are, however, limited^1–5. In addition, there are only two reports on in vitro amino-acid incorporating systems developed from mycelial fungi^6,7. This note briefly reports on the preparation of an in vitro amino-acid incorporating system from the mycelial fungus, Penicillium cyclopium, and the interchangeability of its ribosomes and supernatant enzymes with those prepared from a bacterium, Escherichia coli, a yeast, Saccharomyces cerevisiae, and a higher plant, seedlings of castor bean (Ricinus communis).